Interactions of phospholipid bilayer vesicles with monomolecular films at the air-water interface

Vitthal S. Kulkarni, Rhoderick E Brown

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We report on interactions between a monomolecular film of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) and bilayer vesicles of POPC suspended in the subphase of a Langmuir film balance. A large decrease in area of the monolayer held at a constant surface pressure π of 30 mN m-1 was observed in the presence of either small or large unilamellar vesicles. By using [14C]-POPC, we found that there was no appreciable lipid transfer from vesicle to monolayer or vice versa. The decrease in area of the monolayer held at π=30 mN m-1 is attributed to a localized reorganization of lipid in the film due to the transient collisional contacts between subphase vesicles and monolayer without significant transfer of lipid. POPC monolayers held at constant surface pressures π of 15 and 20 mN m-1 did not show area decreases in the presence of vesicles. This indicated that the transient collisional contacts between vesicles and monolayer did not cause localized reorganization of lipid in the film when the monolayer was held at π=20 mN m-1. We also found that the size of the vesicles had little influence on the interactions between monolayer and vesicles. In contrast, the physicochemical nature of the Teflon surface strongly influences the behavior of the system.

Original languageEnglish (US)
Pages (from-to)869-873
Number of pages5
JournalThin Solid Films
Volume244
Issue number1-2
DOIs
StatePublished - May 15 1994

Fingerprint Dive into the research topics of 'Interactions of phospholipid bilayer vesicles with monomolecular films at the air-water interface'. Together they form a unique fingerprint.

Cite this