Interactions between the Microtubule Binding Protein EB1 and F-Actin

Emily O. Alberico, Zhiqing C. Zhu, Yueh Fu O. Wu, Melissa K Gardner, Dave R. Kovar, Holly V. Goodson

Research output: Contribution to journalArticle

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Abstract

Many cellular processes including cell division and cell migration require coordination between the actin and microtubule (MT) cytoskeletons. This coordination is as-yet poorly understood, but proteins such as formins and IQGAP1 are known to be involved. We show that the MT binding protein EB1 (end-binding protein 1), a key regulator of MT dynamics, can bind directly to filamentous actin (F-actin) F-actin. We determined that the EB1:F-actin interaction is salt sensitive and weak under physiological salt concentrations but might be relevant in contexts where the local concentration of actin is high. Using bioinformatics and mutagenesis, we found that the EB1:F-actin binding site partially overlaps the well-characterized EB1:MT binding interface. Congruently, competition experiments indicate that EB1 can bind to F-actin or MTs but not both simultaneously. These observations suggest that EB1:F-actin interactions may negatively regulate EB1:MT interactions, and we speculate that this interaction may assist cells in differentially regulating MT stability in the actin-rich cortex as opposed to the cell interior.

Original languageEnglish (US)
Pages (from-to)1304-1314
Number of pages11
JournalJournal of Molecular Biology
Volume428
Issue number6
DOIs
StatePublished - Mar 27 2016

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Microtubule Proteins
Protein Binding
Actins
Carrier Proteins
Microtubules
Salts
Computational Biology
Actin Cytoskeleton
Mutagenesis
Cell Division
Cell Movement
Binding Sites

Keywords

  • EB1
  • actin
  • cytoskeleton
  • microtubules
  • plus-end tracking protein

Cite this

Alberico, E. O., Zhu, Z. C., Wu, Y. F. O., Gardner, M. K., Kovar, D. R., & Goodson, H. V. (2016). Interactions between the Microtubule Binding Protein EB1 and F-Actin. Journal of Molecular Biology, 428(6), 1304-1314. https://doi.org/10.1016/j.jmb.2016.01.032

Interactions between the Microtubule Binding Protein EB1 and F-Actin. / Alberico, Emily O.; Zhu, Zhiqing C.; Wu, Yueh Fu O.; Gardner, Melissa K; Kovar, Dave R.; Goodson, Holly V.

In: Journal of Molecular Biology, Vol. 428, No. 6, 27.03.2016, p. 1304-1314.

Research output: Contribution to journalArticle

Alberico, EO, Zhu, ZC, Wu, YFO, Gardner, MK, Kovar, DR & Goodson, HV 2016, 'Interactions between the Microtubule Binding Protein EB1 and F-Actin', Journal of Molecular Biology, vol. 428, no. 6, pp. 1304-1314. https://doi.org/10.1016/j.jmb.2016.01.032
Alberico, Emily O. ; Zhu, Zhiqing C. ; Wu, Yueh Fu O. ; Gardner, Melissa K ; Kovar, Dave R. ; Goodson, Holly V. / Interactions between the Microtubule Binding Protein EB1 and F-Actin. In: Journal of Molecular Biology. 2016 ; Vol. 428, No. 6. pp. 1304-1314.
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