Interactions between PD-1 and PD-L1 promote tolerance by blocking the TCR-induced stop signal.

Brian T. Fife, Kristen E. Pauken, Todd N. Eagar, Takashi Obu, Jenny Wu, Qizhi Tang, Miyuki Azuma, Matthew F. Krummel, Jeffrey A. Bluestone

Research output: Contribution to journalArticle

446 Scopus citations

Abstract

Programmed death 1 (PD-1) is an inhibitory molecule expressed on activated T cells; however, the biological context in which PD-1 controls T cell tolerance remains unclear. Using two-photon laser-scanning microscopy, we show here that unlike naive or activated islet antigen-specific T cells, tolerized islet antigen-specific T cells moved freely and did not swarm around antigen-bearing dendritic cells (DCs) in pancreatic lymph nodes. Inhibition of T cell antigen receptor (TCR)-driven stop signals depended on continued interactions between PD-1 and its ligand, PD-L1, as antibody blockade of PD-1 or PD-L1 resulted in lower T cell motility, enhanced T cell-DC contacts and caused autoimmune diabetes. Blockade of the immunomodulatory receptor CTLA-4 did not alter T cell motility or abrogate tolerance. Thus, PD-1-PD-L1 interactions maintain peripheral tolerance by mechanisms fundamentally distinct from those of CTLA-4.

Original languageEnglish (US)
Pages (from-to)1185-1192
Number of pages8
JournalNature immunology
Volume10
Issue number11
DOIs
StatePublished - Nov 2009

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    Fife, B. T., Pauken, K. E., Eagar, T. N., Obu, T., Wu, J., Tang, Q., Azuma, M., Krummel, M. F., & Bluestone, J. A. (2009). Interactions between PD-1 and PD-L1 promote tolerance by blocking the TCR-induced stop signal. Nature immunology, 10(11), 1185-1192. https://doi.org/10.1038/ni.1790