Interaction of vitamin K dependent proteins with membranes

Gary L. Nelsestuen, Walter Kisiel, Richard G. Di Scipio

Research output: Contribution to journalArticlepeer-review

164 Scopus citations


The membrane-binding characteristics of six vitamin K dependent plasma proteins, which have homologous amino acid sequences, were compared. All of these proteins display calcium-dependent membrane binding and the identified equilibria for protein-membrane binding are qualitatively the same for all proteins. Quantitative characteristics of these protein-membrane interactions allow organization into distinct subgroups. Protein C and factor VII form a subgroup which has extremely low affinity for bilayer membranes; prothrombin, factor X, and protein S form the tightest complexes with membranes and factor IX displays intermediate affinity. In the presence of manganese (which substitutes for calcium in a cation-dependent protein transition), calcium titration of protein-membrane binding shows the same calcium dependence for all proteins except prothrombin which requires lower calcium. These protein-membrane binding characteristics agree very well with the relatedness of these proteins based on their partial amino-terminal sequences.

Original languageEnglish (US)
Pages (from-to)2134-2138
Number of pages5
Issue number11
StatePublished - 1978


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