Interaction of rifabutin with model membranes

Yu N. Shakina; V. V. Vostrikov; G. M. Sorokoumova; A. A. Selishcheva; V. I. Shvets

doi:10.1007/s10517-006-0062-y

Interaction of rifabutin with model membranes

Yu N. Shakina, V. V. Vostrikov, G. M. Sorokoumova, A. A. Selishcheva, V. I. Shvets

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.

Original language	English (US)
Pages (from-to)	711-713
Number of pages	3
Journal	Bulletin of Experimental Biology and Medicine
Volume	140
Issue number	6
DOIs	https://doi.org/10.1007/s10517-006-0062-y
State	Published - Dec 2005

Keywords

Anthryl phosphatidylcholine
Fluorescence technique
Liposomes
Phospholipids
Rifabutin

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10.1007/s10517-006-0062-y

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abstract = "Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.",

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author = "Shakina, {Yu N.} and Vostrikov, {V. V.} and Sorokoumova, {G. M.} and Selishcheva, {A. A.} and Shvets, {V. I.}",

year = "2005",

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T1 - Interaction of rifabutin with model membranes

AU - Shakina, Yu N.

AU - Vostrikov, V. V.

AU - Sorokoumova, G. M.

AU - Selishcheva, A. A.

AU - Shvets, V. I.

PY - 2005/12

Y1 - 2005/12

N2 - Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.

AB - Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.

KW - Anthryl phosphatidylcholine

KW - Fluorescence technique

KW - Liposomes

KW - Phospholipids

KW - Rifabutin

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JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

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ER -

Interaction of rifabutin with model membranes

Abstract

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