Interaction of rifabutin with model membranes

Yu N. Shakina, V. V. Vostrikov, G. M. Sorokoumova, A. A. Selishcheva, V. I. Shvets

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.

Original languageEnglish (US)
Pages (from-to)711-713
Number of pages3
JournalBulletin of Experimental Biology and Medicine
Volume140
Issue number6
DOIs
StatePublished - Dec 1 2005

Keywords

  • Anthryl phosphatidylcholine
  • Fluorescence technique
  • Liposomes
  • Phospholipids
  • Rifabutin

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