Interaction of Erp protein of Mycobacterium tuberculosis with Rv2212 enhances intracellular survival of Mycobacterium smegmatis

Arsheed Ahmad Ganaie, Garima Trivedi, Amanpreet Kaur, Sidharth Shankar Jha, Shashi Anand, Vibhuti Rana, Amit Singh, Shekhar Kumar, Charu Sharma

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo. Although attempts have been made to understand the function of Erp protein, its exact role in Mycobacterium pathogenesis is still elusive. One way to determine this is by searching for novel interactions of RvErp. Using a yeast two-hybrid assay, an adenylyl cyclase (AC), Rv2212, was found to interact with RvErp. The interaction between RvErp and Rv2212 is direct and occurs at the endogenous level. The Erp protein of Mycobacterium smegmatis (MSMEG_6405, or MsErp) interacts neither with Rv2212 nor with Ms_4279, the M. smegmatis homologue of Rv2212. Deletion mutants of Rv2212 revealed its adenylyl cyclase domain to be responsible for the interaction. RvErp enhances Rv2212-mediated cyclic AMP (cAMP) production. Also, the biological significance of the interaction between RvErp and Rv2212 was demonstrated by the enhanced survival of M. smegmatis within THP-1 macrophages. Taken together, these studies address a novel mechanism by which Erp executes its function.

Original languageEnglish (US)
Pages (from-to)2841-2852
Number of pages12
JournalJournal of bacteriology
Volume198
Issue number20
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016, American Society for Microbiology. All Rights Reserved.

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