Interaction between prostaglandin E1 and forskolin in modulation of cyclic AMP levels in rat epididymal adipocytes

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Abstract

The interaction between the diterpene, forskolin and prostaglandin E1l on cAMP levels in isolated adipocytes of the rat was examined. Various doses of Prostaglandin E1 in the presence of 1-methyl-3-isobutyl xanthine (2 mM) blocked the increase in the intracellular level of CAMP elicited by forskolin (0.1 PM or 5 PM). Interestingly, in the absence of 1-methyl-3-isobutylxanthine (or an adenosine blocker), or in the presence of 1-methyl-3-isobutylxanthine (2 mM) and forskolin (30 PM), prostaglandin E1 (1 nM-100 μM) exhibited biphasic dose-response kinetics. The stimulatory effect of PGE1 (> 1 μM) in presence of forskolin (30 μM may be a consequence of prostaglandin receptor modification following endogeneous release of adenosine and prostaglandins by adipocytes stimulated to high levels of cAMP accumulation due to 30 PM forskolin.

Original languageEnglish (US)
Pages (from-to)151-161
Number of pages11
JournalProstaglandins, Leukotrienes and Medicine
Volume18
Issue number2
DOIs
StatePublished - May 1985

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