Integrated transcriptional and phytochemical analyses of the flavonoid biosynthesis pathway in Epimedium

Shaohua Zeng; Yilan Liu; Weiming Hu; Yongliang Liu; Xiaofei Shen; Ying Wang

doi:10.1007/s11240-013-0367-2

Integrated transcriptional and phytochemical analyses of the flavonoid biosynthesis pathway in Epimedium

Shaohua Zeng, Yilan Liu, Weiming Hu, Yongliang Liu, Xiaofei Shen, Ying Wang

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Epimedium is a widely used traditional Chinese medicinal plant due to its high content of health-promoting components such as epimedin A, epimedin B, epimedin C, and icariin (hereafter designated as bioactive compounds); however, the biosynthesis of these bioactive compounds in Epimedium remains to be elucidated. In this study, E. sagitattum flavonoid genes, including three copies of chalcone synthase, two copies of dihydroflavonol 4-reductase, and a single copy of each of chalcone isomerase, flavanone 3 hydroxylase, flavonol synthase, and anthocyanidin synthase, have been isolated. In addition, several transcription factors involved in flavonol, anthocyanin, and proanthocyanin pathways, were identified from an Epimedium 454 EST database. Gene expression profiles and the levels of bioactive compounds in light-stressed E. pubescens leaves in response to phytohormones [abscisic acid (ABA), gibberellic acid (GA₃), indole-3-acetic acid (IAA), salicylic acid (SA)] and sucrose were investigated. All bioactive compounds were significantly elevated by phytohormone and sucrose application except for epimedin B in SA-treated samples and epimedin C following GA₃ and sucrose treatment. Our results suggest that phytohormone and sucrose treatments offer a method for promoting the accumulation of bioactive compounds in Epimedium.

Original language	English (US)
Pages (from-to)	355-365
Number of pages	11
Journal	Plant Cell, Tissue and Organ Culture
Volume	115
Issue number	3
DOIs	https://doi.org/10.1007/s11240-013-0367-2
State	Published - Dec 2013
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the National Natural Science Foundation of China (31270340, 31200225, and 30800624), the South China Botanical Garden Startup Fund (201039), the Knowledge Innovation Project of the Chinese Academy of Sciences (KSCX2-EW-J-20), and the CAS/SAFEA International Partnership Program for Creative Research Teams Project. We thank Dr. Alice Hayward for her help in polishing our manuscript. We also appreciate Dr. Xiang Gao for his help in experiment.

Keywords

Epimedium
Flavonoid biosynthesis
Light stress
Phytohormone
Sucrose

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10.1007/s11240-013-0367-2

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title = "Integrated transcriptional and phytochemical analyses of the flavonoid biosynthesis pathway in Epimedium",

abstract = "Epimedium is a widely used traditional Chinese medicinal plant due to its high content of health-promoting components such as epimedin A, epimedin B, epimedin C, and icariin (hereafter designated as bioactive compounds); however, the biosynthesis of these bioactive compounds in Epimedium remains to be elucidated. In this study, E. sagitattum flavonoid genes, including three copies of chalcone synthase, two copies of dihydroflavonol 4-reductase, and a single copy of each of chalcone isomerase, flavanone 3 hydroxylase, flavonol synthase, and anthocyanidin synthase, have been isolated. In addition, several transcription factors involved in flavonol, anthocyanin, and proanthocyanin pathways, were identified from an Epimedium 454 EST database. Gene expression profiles and the levels of bioactive compounds in light-stressed E. pubescens leaves in response to phytohormones [abscisic acid (ABA), gibberellic acid (GA3), indole-3-acetic acid (IAA), salicylic acid (SA)] and sucrose were investigated. All bioactive compounds were significantly elevated by phytohormone and sucrose application except for epimedin B in SA-treated samples and epimedin C following GA3 and sucrose treatment. Our results suggest that phytohormone and sucrose treatments offer a method for promoting the accumulation of bioactive compounds in Epimedium.",

keywords = "Epimedium, Flavonoid biosynthesis, Light stress, Phytohormone, Sucrose",

author = "Shaohua Zeng and Yilan Liu and Weiming Hu and Yongliang Liu and Xiaofei Shen and Ying Wang",

note = "Funding Information: Acknowledgments This work was supported by the National Natural Science Foundation of China (31270340, 31200225, and 30800624), the South China Botanical Garden Startup Fund (201039), the Knowledge Innovation Project of the Chinese Academy of Sciences (KSCX2-EW-J-20), and the CAS/SAFEA International Partnership Program for Creative Research Teams Project. We thank Dr. Alice Hayward for her help in polishing our manuscript. We also appreciate Dr. Xiang Gao for his help in experiment.",

year = "2013",

month = dec,

doi = "10.1007/s11240-013-0367-2",

language = "English (US)",

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T1 - Integrated transcriptional and phytochemical analyses of the flavonoid biosynthesis pathway in Epimedium

AU - Zeng, Shaohua

AU - Liu, Yilan

AU - Hu, Weiming

AU - Liu, Yongliang

AU - Shen, Xiaofei

AU - Wang, Ying

N1 - Funding Information: Acknowledgments This work was supported by the National Natural Science Foundation of China (31270340, 31200225, and 30800624), the South China Botanical Garden Startup Fund (201039), the Knowledge Innovation Project of the Chinese Academy of Sciences (KSCX2-EW-J-20), and the CAS/SAFEA International Partnership Program for Creative Research Teams Project. We thank Dr. Alice Hayward for her help in polishing our manuscript. We also appreciate Dr. Xiang Gao for his help in experiment.

PY - 2013/12

Y1 - 2013/12

N2 - Epimedium is a widely used traditional Chinese medicinal plant due to its high content of health-promoting components such as epimedin A, epimedin B, epimedin C, and icariin (hereafter designated as bioactive compounds); however, the biosynthesis of these bioactive compounds in Epimedium remains to be elucidated. In this study, E. sagitattum flavonoid genes, including three copies of chalcone synthase, two copies of dihydroflavonol 4-reductase, and a single copy of each of chalcone isomerase, flavanone 3 hydroxylase, flavonol synthase, and anthocyanidin synthase, have been isolated. In addition, several transcription factors involved in flavonol, anthocyanin, and proanthocyanin pathways, were identified from an Epimedium 454 EST database. Gene expression profiles and the levels of bioactive compounds in light-stressed E. pubescens leaves in response to phytohormones [abscisic acid (ABA), gibberellic acid (GA3), indole-3-acetic acid (IAA), salicylic acid (SA)] and sucrose were investigated. All bioactive compounds were significantly elevated by phytohormone and sucrose application except for epimedin B in SA-treated samples and epimedin C following GA3 and sucrose treatment. Our results suggest that phytohormone and sucrose treatments offer a method for promoting the accumulation of bioactive compounds in Epimedium.

AB - Epimedium is a widely used traditional Chinese medicinal plant due to its high content of health-promoting components such as epimedin A, epimedin B, epimedin C, and icariin (hereafter designated as bioactive compounds); however, the biosynthesis of these bioactive compounds in Epimedium remains to be elucidated. In this study, E. sagitattum flavonoid genes, including three copies of chalcone synthase, two copies of dihydroflavonol 4-reductase, and a single copy of each of chalcone isomerase, flavanone 3 hydroxylase, flavonol synthase, and anthocyanidin synthase, have been isolated. In addition, several transcription factors involved in flavonol, anthocyanin, and proanthocyanin pathways, were identified from an Epimedium 454 EST database. Gene expression profiles and the levels of bioactive compounds in light-stressed E. pubescens leaves in response to phytohormones [abscisic acid (ABA), gibberellic acid (GA3), indole-3-acetic acid (IAA), salicylic acid (SA)] and sucrose were investigated. All bioactive compounds were significantly elevated by phytohormone and sucrose application except for epimedin B in SA-treated samples and epimedin C following GA3 and sucrose treatment. Our results suggest that phytohormone and sucrose treatments offer a method for promoting the accumulation of bioactive compounds in Epimedium.

KW - Epimedium

KW - Flavonoid biosynthesis

KW - Light stress

KW - Phytohormone

KW - Sucrose

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M3 - Article

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SN - 0167-6857

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Integrated transcriptional and phytochemical analyses of the flavonoid biosynthesis pathway in Epimedium

Abstract

Bibliographical note

Keywords

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