Integrated Nanogap Platform for Sub-Volt Dielectrophoretic Trapping and Real-Time Raman Imaging of Biological Nanoparticles

Christopher T. Ertsgaard; Nathan J. Wittenberg; Daniel J. Klemme; Avijit Barik; Wei Chuan Shih; Sang Hyun Oh

doi:10.1021/acs.nanolett.8b02654

Integrated Nanogap Platform for Sub-Volt Dielectrophoretic Trapping and Real-Time Raman Imaging of Biological Nanoparticles

Christopher T. Ertsgaard, Nathan J. Wittenberg, Daniel J. Klemme, Avijit Barik, Wei Chuan Shih, Sang Hyun Oh

Electrical and Computer Engineering

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

A rapid, label-free, and broadly applicable chemical analysis platform for nanovesicles and subcellular components is highly desirable for diagnostic assays. We demonstrate an integrated nanogap plasmonic sensing platform that combines subvolt dielectrophoresis (DEP) trapping, gold nanoparticles (AuNPs), and a lineated illumination scheme for real-time, surface-enhanced Raman spectroscopy (SERS) imaging of biological nanoparticles. Our system is capable of isolating suspended sub-100 nm vesicles and imaging the Raman spectra of their cargo within seconds, 100 times faster than conventional point-scan Raman systems. Bare AuNPs are spiked into solution and simultaneously trapped with the nanovesicles along the gap to boost local optical fields. In addition, our platform offers simultaneous and delay-free spatial and temporal multiplexing functionality. These nanogap devices can be mass-produced via atomic layer lithography and provide a practical platform for high-speed SERS analysis of biological nanoparticles.

Original language	English (US)
Pages (from-to)	5946-5953
Number of pages	8
Journal	Nano letters
Volume	18
Issue number	9
DOIs	https://doi.org/10.1021/acs.nanolett.8b02654
State	Published - Sep 12 2018

Bibliographical note

Funding Information:
This work was supported by grants from the National Science Foundation (NSF ECCS Grant No. 1610333 to C.T.E. and S.-H.O.; NSF CBET Grant No. 1605683 to W.-C.S.) and the Minnesota Partnership for Biotechnology and Medical Genomics (A.B., N.J.W., and S.-H.O.). C.T.E. and D.J.K. acknowledge support from the NSF Graduate Research Fellowship Program (GRFP) and A.B. acknowledges support from the University of Minnesota Doctoral Dissertation Fellowship. N.J.W. acknowledges support from Lehigh University. Device fabrication was performed at the University of Minnesota Nanofabrication Center, which receives support from the NSF through the National Nanotechnology Coordinated Infrastructure (NNCI) program.

Publisher Copyright:
© 2018 American Chemical Society.

Keywords

Dielectrophoresis
Raman
gold nanoparticle
nanogap
surface-enhanced Raman scattering (SERS)
vesicle

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10.1021/acs.nanolett.8b02654

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title = "Integrated Nanogap Platform for Sub-Volt Dielectrophoretic Trapping and Real-Time Raman Imaging of Biological Nanoparticles",

abstract = "A rapid, label-free, and broadly applicable chemical analysis platform for nanovesicles and subcellular components is highly desirable for diagnostic assays. We demonstrate an integrated nanogap plasmonic sensing platform that combines subvolt dielectrophoresis (DEP) trapping, gold nanoparticles (AuNPs), and a lineated illumination scheme for real-time, surface-enhanced Raman spectroscopy (SERS) imaging of biological nanoparticles. Our system is capable of isolating suspended sub-100 nm vesicles and imaging the Raman spectra of their cargo within seconds, 100 times faster than conventional point-scan Raman systems. Bare AuNPs are spiked into solution and simultaneously trapped with the nanovesicles along the gap to boost local optical fields. In addition, our platform offers simultaneous and delay-free spatial and temporal multiplexing functionality. These nanogap devices can be mass-produced via atomic layer lithography and provide a practical platform for high-speed SERS analysis of biological nanoparticles.",

keywords = "Dielectrophoresis, Raman, gold nanoparticle, nanogap, surface-enhanced Raman scattering (SERS), vesicle",

author = "Ertsgaard, {Christopher T.} and Wittenberg, {Nathan J.} and Klemme, {Daniel J.} and Avijit Barik and Shih, {Wei Chuan} and Oh, {Sang Hyun}",

note = "Funding Information: This work was supported by grants from the National Science Foundation (NSF ECCS Grant No. 1610333 to C.T.E. and S.-H.O.; NSF CBET Grant No. 1605683 to W.-C.S.) and the Minnesota Partnership for Biotechnology and Medical Genomics (A.B., N.J.W., and S.-H.O.). C.T.E. and D.J.K. acknowledge support from the NSF Graduate Research Fellowship Program (GRFP) and A.B. acknowledges support from the University of Minnesota Doctoral Dissertation Fellowship. N.J.W. acknowledges support from Lehigh University. Device fabrication was performed at the University of Minnesota Nanofabrication Center, which receives support from the NSF through the National Nanotechnology Coordinated Infrastructure (NNCI) program. Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

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AU - Barik, Avijit

AU - Shih, Wei Chuan

AU - Oh, Sang Hyun

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PY - 2018/9/12

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N2 - A rapid, label-free, and broadly applicable chemical analysis platform for nanovesicles and subcellular components is highly desirable for diagnostic assays. We demonstrate an integrated nanogap plasmonic sensing platform that combines subvolt dielectrophoresis (DEP) trapping, gold nanoparticles (AuNPs), and a lineated illumination scheme for real-time, surface-enhanced Raman spectroscopy (SERS) imaging of biological nanoparticles. Our system is capable of isolating suspended sub-100 nm vesicles and imaging the Raman spectra of their cargo within seconds, 100 times faster than conventional point-scan Raman systems. Bare AuNPs are spiked into solution and simultaneously trapped with the nanovesicles along the gap to boost local optical fields. In addition, our platform offers simultaneous and delay-free spatial and temporal multiplexing functionality. These nanogap devices can be mass-produced via atomic layer lithography and provide a practical platform for high-speed SERS analysis of biological nanoparticles.

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Integrated Nanogap Platform for Sub-Volt Dielectrophoretic Trapping and Real-Time Raman Imaging of Biological Nanoparticles

Abstract

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Keywords

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