Insulin-like growth factor mRNA expression in tissues of lean and obese male SHFF/Mcc-facp rats

Liesl De Sevilla; Sylvia A. McCune; Michael E. White

doi:10.1016/0305-0491(94)90020-5

Insulin-like growth factor mRNA expression in tissues of lean and obese male SHFF/Mcc-fa^cp rats

Liesl De Sevilla
, Sylvia A. McCune
, Michael E. White

Administration (CFANS)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

No differences were detected in serum IGF-I levels between lean and obese male SHFF/Mcc-fa^cp rats expressing non-insulin-dependent diabetes mellitus (NIDDM). In contrast, serum insulin levels, and blood glucose levels were significantly elevated in obese as compared to lean littermates (P < 0.05), indicating that the obese animals were diabetic. Northern blot analyses of total tissue RNA using labeled cDNAs for IGF-I and IGF-II revealed a decrease in liver and adipose IGF-I mRNA expression in the obese littermates while IGF-II mRNA expression was decreased only in adipose tissue of obese animals as compared to lean littermates.

Original language	English (US)
Pages (from-to)	371-382
Number of pages	12
Journal	Comparative biochemistry and physiology. B, Comparative biochemistry
Volume	109
Issue number	2-3
DOIs	https://doi.org/10.1016/0305-0491(94)90020-5
State	Published - 1994

Bibliographical note

Funding Information:
Acknowledgements--This work was supported by an Interdisciplinary Grant from The Ohio State University and by Clinical Research Center Grant MO1-RR-OO034. Salaries and research support provided by the state and federal funds appropriated to the Ohio Agricultural Research and Development Center and The Ohio State University, Manuscript No. 224-93. We thank Dr Thomas M. O'Dorisio, M.D., and his laboratory for performing the insulin RIA and Jeanne Osborne, Kim Kampman, Jody Duffy, Dr Mike Sherman and Dr Tim Ramsay for their support and technical assistance. We would also like to especially thank Catherine Ernst for her excellent technical support and advice.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Corpulent rat
IGF-1
IGF-2
Insulin-like growth factors
Obesity
mRNA

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10.1016/0305-0491(94)90020-5

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@article{983242bd197d4451b553eaf29946dbec,

title = "Insulin-like growth factor mRNA expression in tissues of lean and obese male SHFF/Mcc-facp rats",

abstract = "No differences were detected in serum IGF-I levels between lean and obese male SHFF/Mcc-facp rats expressing non-insulin-dependent diabetes mellitus (NIDDM). In contrast, serum insulin levels, and blood glucose levels were significantly elevated in obese as compared to lean littermates (P < 0.05), indicating that the obese animals were diabetic. Northern blot analyses of total tissue RNA using labeled cDNAs for IGF-I and IGF-II revealed a decrease in liver and adipose IGF-I mRNA expression in the obese littermates while IGF-II mRNA expression was decreased only in adipose tissue of obese animals as compared to lean littermates.",

keywords = "Corpulent rat, IGF-1, IGF-2, Insulin-like growth factors, Obesity, mRNA",

author = "\{De Sevilla\}, Liesl and McCune, \{Sylvia A.\} and White, \{Michael E.\}",

note = "Funding Information: Acknowledgements--This work was supported by an Interdisciplinary Grant from The Ohio State University and by Clinical Research Center Grant MO1-RR-OO034. Salaries and research support provided by the state and federal funds appropriated to the Ohio Agricultural Research and Development Center and The Ohio State University, Manuscript No. 224-93. We thank Dr Thomas M. O'Dorisio, M.D., and his laboratory for performing the insulin RIA and Jeanne Osborne, Kim Kampman, Jody Duffy, Dr Mike Sherman and Dr Tim Ramsay for their support and technical assistance. We would also like to especially thank Catherine Ernst for her excellent technical support and advice.",

year = "1994",

doi = "10.1016/0305-0491(94)90020-5",

language = "English (US)",

volume = "109",

pages = "371--382",

journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",

issn = "0305-0491",

number = "2-3",

}

TY - JOUR

T1 - Insulin-like growth factor mRNA expression in tissues of lean and obese male SHFF/Mcc-facp rats

AU - De Sevilla, Liesl

AU - McCune, Sylvia A.

AU - White, Michael E.

N1 - Funding Information: Acknowledgements--This work was supported by an Interdisciplinary Grant from The Ohio State University and by Clinical Research Center Grant MO1-RR-OO034. Salaries and research support provided by the state and federal funds appropriated to the Ohio Agricultural Research and Development Center and The Ohio State University, Manuscript No. 224-93. We thank Dr Thomas M. O'Dorisio, M.D., and his laboratory for performing the insulin RIA and Jeanne Osborne, Kim Kampman, Jody Duffy, Dr Mike Sherman and Dr Tim Ramsay for their support and technical assistance. We would also like to especially thank Catherine Ernst for her excellent technical support and advice.

PY - 1994

Y1 - 1994

N2 - No differences were detected in serum IGF-I levels between lean and obese male SHFF/Mcc-facp rats expressing non-insulin-dependent diabetes mellitus (NIDDM). In contrast, serum insulin levels, and blood glucose levels were significantly elevated in obese as compared to lean littermates (P < 0.05), indicating that the obese animals were diabetic. Northern blot analyses of total tissue RNA using labeled cDNAs for IGF-I and IGF-II revealed a decrease in liver and adipose IGF-I mRNA expression in the obese littermates while IGF-II mRNA expression was decreased only in adipose tissue of obese animals as compared to lean littermates.

AB - No differences were detected in serum IGF-I levels between lean and obese male SHFF/Mcc-facp rats expressing non-insulin-dependent diabetes mellitus (NIDDM). In contrast, serum insulin levels, and blood glucose levels were significantly elevated in obese as compared to lean littermates (P < 0.05), indicating that the obese animals were diabetic. Northern blot analyses of total tissue RNA using labeled cDNAs for IGF-I and IGF-II revealed a decrease in liver and adipose IGF-I mRNA expression in the obese littermates while IGF-II mRNA expression was decreased only in adipose tissue of obese animals as compared to lean littermates.

KW - Corpulent rat

KW - IGF-1

KW - IGF-2

KW - Insulin-like growth factors

KW - Obesity

KW - mRNA

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U2 - 10.1016/0305-0491(94)90020-5

DO - 10.1016/0305-0491(94)90020-5

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AN - SCOPUS:0027971003

SN - 0305-0491

VL - 109

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EP - 382

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

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