Insulin-like growth factor I expression by tumors of neuroectodermal origin with the t(11;22) chromosomal translocation. A potential autocrine growth factor

D. Yee, R. E. Favoni, G. S. Lebovic, F. Lombana, D. R. Powell, C. P. Reynolds, N. Rosen

Research output: Contribution to journalArticlepeer-review

154 Scopus citations

Abstract

Expression of insulin-like growth factor I (IGF-I) mRNA by some tumor cell lines of neuroectodermal origin has been described. To further explore the significance of IGF-I mRNA expression in these tumors, a more extensive analysis was performed. Most (9 of 10) neuroectodermal tumor cell lines with a t(11;22) translocation (primitive neuroectodermal tumor [PNET], Ewing's sarcoma, esthesioneuroblastoma) expressed IGF-I mRNA, whereas 0 of 15 cell lines without the translocation (PNET, neuroblastoma) expressed IGF-I. Furthermore, inasmuch as all neuroblastoma (12 of 12) cell lines examined expressed IGF-II RNA, the pattern of IGF expression could distinguish between these closely related tumors. CHP-100, a PNET cell line with the t(11;22) translocation, was shown to secrete both IGF-I protein and an IGF binding protein, IGFBP-2. This cell line also expressed the type I IGF receptor mRNA, and blockade of this receptor by a monoclonal antibody (αIR3) inhibited serum-free growth. These data demonstrate that IGF-I expression is a property of neuroectodermal tumors with a t(11;22) translocation and that interruption of an IGF-I autocrine loop inhibits the growth of these tumor cells.

Original languageEnglish (US)
Pages (from-to)1806-1814
Number of pages9
JournalJournal of Clinical Investigation
Volume86
Issue number6
DOIs
StatePublished - 1990

Keywords

  • Ewing's sarcoma
  • insulin-like growth factor II
  • insulin-like growth factor binding protein 2
  • neuroblastoma
  • type I insulin-like growth factor receptor

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