Insulin-like growth factor binding protein 1 expression inhibits insulin- like growth factor I action in MCF-7 breast cancer cells

D. Yee, J. G. Jackson, T. W. Kozelsky, J. A. Figueroa

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Abstract

Interactions between insulin-like growth factor I (IGF-I) and the type I IGF receptor may be affected by high-affinity extracellular binding proteins. To date, six distinct IGF binding proteins (IGFBPs) have been identified, but their physiological roles are not well understood. For example, depending on experimental conditions, IGFBP-1 has been shown to both enhance and inhibit IGF-I mediated mitogenesis. We have previously shown that excess exogenous IGFBP-1 inhibited IGF-I mediated growth of MCF-7 cells. In this study, we examined whether or not endogenously expressed IGFBP-1 could interfere with IGF-I mediated growth of MCF-7 cells. Cells were stably transfected with an IGFBP-1 expression vector. IGFBP-1 mRNA was produced by the cells, and protein was detected in the conditioned media by ligand blot and immunoblot. Type I IGF receptor could not be phosphorylated by IGF-I in cells expressing IGFBP-1; however, an IGF-I analogue (Arg-3-IGF-I), which cannot complex with IGFBPs, stimulated receptor phosphorylation. IGF-I did not stimulate cell growth in IGFBP-1 expressing cells. These results suggest that IGFBP-1 expression in MCF-7 breast cancer cells inhibits IGF-I induced growth by interrupting the interaction between IGF-I and its receptor.

Original languageEnglish (US)
Pages (from-to)73-77
Number of pages5
JournalCell Growth and Differentiation
Volume5
Issue number1
StatePublished - Jan 1 1994

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Insulin-Like Growth Factor Binding Protein 1
Insulin-Like Growth Factor I
Breast Neoplasms
IGF Type 1 Receptor
Insulin-Like Growth Factor Binding Proteins
MCF-7 Cells
Growth
Conditioned Culture Medium
Carrier Proteins
Phosphorylation
Ligands
Messenger RNA

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Insulin-like growth factor binding protein 1 expression inhibits insulin- like growth factor I action in MCF-7 breast cancer cells. / Yee, D.; Jackson, J. G.; Kozelsky, T. W.; Figueroa, J. A.

In: Cell Growth and Differentiation, Vol. 5, No. 1, 01.01.1994, p. 73-77.

Research output: Contribution to journalArticle

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