Instrumentation for direct microscopic elemental analysis of frozen biological tissue

Thos E. Hutchinson, Marvin Bacaner, John H Broadhurst, John Lilley

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A system has been devised to provide direct observation of frozen biological tissue in the scanning electron microscope. Both transmission and secondary electron methods of signal collection have been obtained. In combination with energy elective fluorescent x-ray analysis, the elemental constituents of subcellular regions less than 1000 Å in diameter have been analyzed in the transmission mode of operation. Transmission scanning electron micrographs of frozen muscle shows significant microstructural differences with conventionally obtained electron micrographs although dark bands, possibly corresponding to z zones of classically observed spacing, are seen.

Original languageEnglish (US)
Pages (from-to)252-255
Number of pages4
JournalReview of Scientific Instruments
Volume45
Issue number2
DOIs
StatePublished - Dec 1 1974

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