Abstract
A system has been devised to provide direct observation of frozen biological tissue in the scanning electron microscope. Both transmission and secondary electron methods of signal collection have been obtained. In combination with energy elective fluorescent x-ray analysis, the elemental constituents of subcellular regions less than 1000 Å in diameter have been analyzed in the transmission mode of operation. Transmission scanning electron micrographs of frozen muscle shows significant microstructural differences with conventionally obtained electron micrographs although dark bands, possibly corresponding to z zones of classically observed spacing, are seen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 252-255 |
| Number of pages | 4 |
| Journal | Review of Scientific Instruments |
| Volume | 45 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1974 |