Insight into the effects of plasmodium chabaudi on platelets using carbon-fiber microelectrode amperometry

Kang Xiong-Hang, Kaila Kemnetz-Ness, Anna C. Krieger, Christy L Haynes

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Platelets are anuclear circulating cell bodies within the bloodstream commonly known for their roles in clot formation during vascular injury to prevent blood loss. They also have significant impact in a range of diseases, including malaria. However, the role of platelets in malaria is controversial, with contradicting evidence suggesting either that they assist in destruction of malarial parasites or facilitate a severe form of malaria. Precedent work suggests that the timing of infection is critical in determining whether platelets switch roles from being protective to deleterious. As such, the work herein makes use of the unique mechanistic perspective offered by carbon-fiber microelectrode amperometry (CFMA) to understand how platelet secretion is impacted in malarial infection stages (ascending parasite count versus descending parasite count). Malarial platelet behavior was compared to platelets from noninfected control mice by probing their exocytotic function. Results suggest that mouse malaria caused by the parasite Plasmodium chabaudi, during both ascending and descending infection stages, reduces platelet exocytotic events and delays platelet granule fusion; in addition, platelets are more impacted by the disease early in the infection stages. In all, understanding platelet behavior in the malarial context may present new therapeutic routes to treat or cure malaria.

Original languageEnglish (US)
Pages (from-to)592-597
Number of pages6
JournalACS Infectious Diseases
Volume5
Issue number4
DOIs
StatePublished - Apr 12 2019

Bibliographical note

Publisher Copyright:
© 2019 American Chemical Society.

Keywords

  • carbon-fiber microelectrode amperometry
  • electrochemical technique
  • infected red blood cells
  • malaria
  • Plasmodium chabaudi
  • platelets
  • single cell

MRSEC Support

  • Shared

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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