TY - JOUR
T1 - Inner ear damage and passage through the round window membrane of Pseudomonas Aeruginosa exotoxin a in a chinchilla model
AU - Lundman, Lars
AU - Harada, Teruhiko
AU - Santi, Peter A
AU - Juhn, Steven K.
AU - Morizono, Tetsuo
AU - Bagger-Sjöbäck, Dan
PY - 1992/5
Y1 - 1992/5
N2 - By the use of computer-assisted morphometric analysis of the organ of Corti and/or measurements of action potential threshold changes, inner ear changes in chinchillas were evaluated 4 weeks after application on the round window membrane of a Pseudomonas aeruginosa exotoxin A solution. Severe inner ear damage was detected after application of 50 ng (5 μL at a concentration of 10 μg/mL) exotoxin A, whereas application of 5 ng exotoxin did not cause measurable inner ear damage. Perilymph concentrations of exotoxin A were measured with an enzyme-linked immunosorbent assay 1.5 to 19 hours after 50 ng, 0.5 μg, or 5 μg of exotoxin A was applied on the round window membrane. Only the highest concentration produced measurable levels of exotoxin in the inner ear fluids. It is concluded that exotoxin A present on the round window membrane of the chinchilla has the ability to penetrate into the inner ear and cause irreversible inner ear changes.
AB - By the use of computer-assisted morphometric analysis of the organ of Corti and/or measurements of action potential threshold changes, inner ear changes in chinchillas were evaluated 4 weeks after application on the round window membrane of a Pseudomonas aeruginosa exotoxin A solution. Severe inner ear damage was detected after application of 50 ng (5 μL at a concentration of 10 μg/mL) exotoxin A, whereas application of 5 ng exotoxin did not cause measurable inner ear damage. Perilymph concentrations of exotoxin A were measured with an enzyme-linked immunosorbent assay 1.5 to 19 hours after 50 ng, 0.5 μg, or 5 μg of exotoxin A was applied on the round window membrane. Only the highest concentration produced measurable levels of exotoxin in the inner ear fluids. It is concluded that exotoxin A present on the round window membrane of the chinchilla has the ability to penetrate into the inner ear and cause irreversible inner ear changes.
KW - Pseudomonas aeruginosa exotoxin A
KW - round window membrane permeability
KW - sensorineural hearing loss
UR - http://www.scopus.com/inward/record.url?scp=0026633780&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026633780&partnerID=8YFLogxK
U2 - 10.1177/000348949210100511
DO - 10.1177/000348949210100511
M3 - Article
C2 - 1570939
AN - SCOPUS:0026633780
SN - 0003-4894
VL - 101
SP - 437
EP - 444
JO - Annals of Otology, Rhinology & Laryngology
JF - Annals of Otology, Rhinology & Laryngology
IS - 5
ER -