Innate Immune Signaling Contributes to Tubular Cell Senescence in the Glis2 Knockout Mouse Model of Nephronophthisis

Heng Jin, Yan Zhang, Dingxiao Liu, Shan Shanshan Wang, Qiong Ding, Prerna Rastogi, Madison Purvis, Angela Wang, Sarah Elhadi, Chongyu Ren, Chao Cao, Yanfen Chai, Peter Igarashi, Anton M. Jetten, Dongmei Lu, Massimo Attanasio

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Nephronophthisis (NPHP), the leading genetic cause of end-stage renal failure in children and young adults, is a group of autosomal recessive diseases characterized by kidney-cyst degeneration and fibrosis for which no therapy is currently available. To date, mutations in >25 genes have been identified as causes of this disease that, in several cases, result in chronic DNA damage in kidney tubular cells. Among such mutations, those in the transcription factor–encoding GLIS2 cause NPHP type 7. Loss of function of mouse Glis2 causes senescence of kidney tubular cells. Senescent cells secrete proinflammatory molecules that induce progressive organ damage through several pathways, among which NF-κB signaling is prevalent. Herein, we show that the NF-κB signaling is active in Glis2 knockout kidney epithelial cells and that genetic inactivation of the toll-like receptor (TLR)/IL-1 receptor or pharmacologic elimination of senescent cells (senolytic therapy) reduces tubule damage, fibrosis, and apoptosis in the Glis2 mouse model of NPHP. Notably, in Glis2, Tlr2 double knockouts, senescence was also reduced and proliferation was increased, suggesting that loss of TLR2 activity improves the regenerative potential of tubular cells in Glis2 knockout kidneys. Our results further suggest that a combination of TLR/IL-1 receptor inhibition and senolytic therapy may delay the progression of kidney disease in NPHP type 7 and other forms of this disease.

Original languageEnglish (US)
Pages (from-to)176-189
Number of pages14
JournalAmerican Journal of Pathology
Issue number1
StatePublished - Jan 2020

Bibliographical note

Funding Information:
Supported by NIH grants 1R01DK090326 (M.A.) and Z01-ES-101585 (A.M.J.); the National Natural Science Foundation of China grant 81871593 (Y.C.); the National Institute of Environmental Health Sciences Intramural Research Program; and the Fundamental Research Funds for the Central Universities, China, grant 3332019127 (H.J.).

Publisher Copyright:
© 2020 American Society for Investigative Pathology


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