Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation

Jung Hoe Kim, Wei-Shou Hu

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalCytotechnology
Volume2
Issue number2
DOIs
StatePublished - May 1 1989

Keywords

  • agitation rate
  • cell culture
  • cell inoculation density
  • growth rate
  • hepatoma
  • microcarrier

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