Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells

Soo Yeong Park; Hye Ja Lee; Weon Jong Yoon; Gyoung Jin Kang; Ji Young Moon; Nam Ho Lee; Se Jae Kim; Hee Kyoung Kang; Eun Sook Yoo

doi:10.1007/BF02978207

Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells

Soo Yeong Park, Hye Ja Lee, Weon Jong Yoon, Gyoung Jin Kang, Ji Young Moon, Nam Ho Lee, Se Jae Kim, Hee Kyoung Kang, Eun Sook Yoo

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides B and C were isolated as the active components. Sseveral inflammatory markers including TNF-α, IL-1β, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides B and C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-α) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides B and C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside B and C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-α and IL-6), iNOS and COX-2.

Original language	English (US)
Pages (from-to)	1244-1250
Number of pages	7
Journal	Archives of Pharmacal Research
Volume	28
Issue number	11
DOIs	https://doi.org/10.1007/BF02978207
State	Published - Nov 2005
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Korea Science and Engineering Foundation Grant (R05-2003-000-10494-0).

Keywords

COX-2
Eutigoside B
Eutigoside C
Pro-inflammatory cytokines
iNOS

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10.1007/BF02978207

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title = "Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells",

abstract = "The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides B and C were isolated as the active components. Sseveral inflammatory markers including TNF-α, IL-1β, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides B and C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-α) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides B and C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside B and C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-α and IL-6), iNOS and COX-2.",

keywords = "COX-2, Eutigoside B, Eutigoside C, Pro-inflammatory cytokines, iNOS",

author = "Park, {Soo Yeong} and Lee, {Hye Ja} and Yoon, {Weon Jong} and Kang, {Gyoung Jin} and Moon, {Ji Young} and Lee, {Nam Ho} and Kim, {Se Jae} and Kang, {Hee Kyoung} and Yoo, {Eun Sook}",

note = "Funding Information: This work was supported by Korea Science and Engineering Foundation Grant (R05-2003-000-10494-0).",

year = "2005",

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doi = "10.1007/BF02978207",

language = "English (US)",

volume = "28",

pages = "1244--1250",

journal = "Archives of Pharmacal Research",

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T1 - Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells

AU - Park, Soo Yeong

AU - Lee, Hye Ja

AU - Yoon, Weon Jong

AU - Kang, Gyoung Jin

AU - Moon, Ji Young

AU - Lee, Nam Ho

AU - Kim, Se Jae

AU - Kang, Hee Kyoung

AU - Yoo, Eun Sook

N1 - Funding Information: This work was supported by Korea Science and Engineering Foundation Grant (R05-2003-000-10494-0).

PY - 2005/11

Y1 - 2005/11

N2 - The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides B and C were isolated as the active components. Sseveral inflammatory markers including TNF-α, IL-1β, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides B and C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-α) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides B and C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside B and C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-α and IL-6), iNOS and COX-2.

AB - The anti-inflammatory activity of Eurya emarginata (Thumb) Makino, of which leaves have been traditionally used to treat ulcers or diuretic in Jeju Island, has been investigated in the present study. Through the phytochemical study from the methanol extract of E. emaginiata, eutigosides B and C were isolated as the active components. Sseveral inflammatory markers including TNF-α, IL-1β, IL-6, NO, iNOS, and COX-2 were examined. Eutigosides B and C potentially inhibited production of pro-inflammatory cytokines (IL-6 and TNF-α) in a dose-dependent manner. Additionally, the intracellular contents of iNOS protein were markedly decreased after treatment with eutigosides B and C. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. These results suggest that eutigoside B and C from E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines (TNF-α and IL-6), iNOS and COX-2.

KW - COX-2

KW - Eutigoside B

KW - Eutigoside C

KW - Pro-inflammatory cytokines

KW - iNOS

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Inhibitory effects of eutigosides isolated from Eurya emarginata on the inflammatory mediators in RAW264.7 cells

Abstract

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Keywords

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