Inhibition of tumor promoter induced transformation by expression of the small GTP-binding protein Rac in JB6 cells

Zigang Dong, Ming Ding, Wei Y.A. Ma, Nancy H. Colburn

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The small GTP-binding protein rac-1 has been reported to be a mediator for tumor promoter, growth factor and serum-induced cell membrane ruffling. We have stably overexpressed rac-1 in JB6 P+ cells. In contrast to rac-1 expressing NIH/3T3 cells, all JB6 transfectants expressing rac-1 did not show tumorigenic transformation (forward progression) but instead showed anti- oncogenic effects (backward progression). Transfectants expressing high levels of rac-1 were blocked for TPA-, EGF- and serum-induced transformation, while transfectants expressing lower levels of rac-1 were completely blocked for EGF- and serum-induced transformation but only partially inhibited for TPA-induced transformation compared with vector control transfectants.

Original languageEnglish (US)
Pages (from-to)157-161
Number of pages5
JournalInternational Journal of Oncology
Volume11
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • Cell transformation
  • Rac
  • Tumor promoter

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