Inhibition of swine microglial cell phagocytosis of Cryptococcus neoformans by femtomolar concentrations of morphine

Grzegorz Sowa; Genya Gekker; Myriam M. Lipovsky; Shuxian Hu; Chun C. Chao; Thomas W Molitor; Phillip K. Peterson

doi:10.1016/S0006-2952(97)00006-3

Inhibition of swine microglial cell phagocytosis of Cryptococcus neoformans by femtomolar concentrations of morphine

Grzegorz Sowa, Genya Gekker, Myriam M. Lipovsky, Shuxian Hu, Chun C. Chao, Thomas W Molitor, Phillip K. Peterson

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Microglia are important immune effector cells within the brain. The phagocytosis of nonopsonized Cryptococcus neoformans by swine microglia was used as an in vitro model for studies on cellular mechanisms of opiate-mediated immunomodulation in the brain. Morphine inhibited potently (IC₅₀ ~10^-16 M) the phagocytosis of C. neoformans by primaty cultures of neonatal pig microglia. The μ opioid agonist Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) also suppressed phagocytosis but with a much lower potency than morphine (IC₅₀ ~ 10^-8 M). The inhibitory effects of morphine and DAMGO were blocked by equimolar concentrations of naloxone and by the selective μ opiate receptor antagonist β-funaltrexamine. Pertussis toxin but not cholera toxin reversed the inhibitory effects of both morphine and DAMGO. Our data suggest that morphine inhibits phagocytosis of C. neoformans by swine microglia via a mechanism involving μ opiate receptors coupled to a pertussis toxin-sensitive G(i)/G(o) protein signaling pathway.

Original language	English (US)
Pages (from-to)	823-828
Number of pages	6
Journal	Biochemical Pharmacology
Volume	53
Issue number	6
DOIs	https://doi.org/10.1016/S0006-2952(97)00006-3
State	Published - Mar 21 1997

Bibliographical note

Funding Information:
This work was supportedi,n part, by National Instituteso f He& Grants DA-04351 and DA-08446.

Keywords

Cryptococcus neoformans
microglia
morphine
phagocytosis
μ opiate receptor

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10.1016/S0006-2952(97)00006-3

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title = "Inhibition of swine microglial cell phagocytosis of Cryptococcus neoformans by femtomolar concentrations of morphine",

abstract = "Microglia are important immune effector cells within the brain. The phagocytosis of nonopsonized Cryptococcus neoformans by swine microglia was used as an in vitro model for studies on cellular mechanisms of opiate-mediated immunomodulation in the brain. Morphine inhibited potently (IC50 ~10-16 M) the phagocytosis of C. neoformans by primaty cultures of neonatal pig microglia. The μ opioid agonist Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) also suppressed phagocytosis but with a much lower potency than morphine (IC50 ~ 10-8 M). The inhibitory effects of morphine and DAMGO were blocked by equimolar concentrations of naloxone and by the selective μ opiate receptor antagonist β-funaltrexamine. Pertussis toxin but not cholera toxin reversed the inhibitory effects of both morphine and DAMGO. Our data suggest that morphine inhibits phagocytosis of C. neoformans by swine microglia via a mechanism involving μ opiate receptors coupled to a pertussis toxin-sensitive G(i)/G(o) protein signaling pathway.",

keywords = "Cryptococcus neoformans, microglia, morphine, phagocytosis, μ opiate receptor",

author = "Grzegorz Sowa and Genya Gekker and Lipovsky, {Myriam M.} and Shuxian Hu and Chao, {Chun C.} and Molitor, {Thomas W} and Peterson, {Phillip K.}",

note = "Funding Information: This work was supportedi,n part, by National Instituteso f He& Grants DA-04351 and DA-08446.",

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doi = "10.1016/S0006-2952(97)00006-3",

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T1 - Inhibition of swine microglial cell phagocytosis of Cryptococcus neoformans by femtomolar concentrations of morphine

AU - Sowa, Grzegorz

AU - Gekker, Genya

AU - Lipovsky, Myriam M.

AU - Hu, Shuxian

AU - Chao, Chun C.

AU - Molitor, Thomas W

AU - Peterson, Phillip K.

N1 - Funding Information: This work was supportedi,n part, by National Instituteso f He& Grants DA-04351 and DA-08446.

PY - 1997/3/21

Y1 - 1997/3/21

N2 - Microglia are important immune effector cells within the brain. The phagocytosis of nonopsonized Cryptococcus neoformans by swine microglia was used as an in vitro model for studies on cellular mechanisms of opiate-mediated immunomodulation in the brain. Morphine inhibited potently (IC50 ~10-16 M) the phagocytosis of C. neoformans by primaty cultures of neonatal pig microglia. The μ opioid agonist Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) also suppressed phagocytosis but with a much lower potency than morphine (IC50 ~ 10-8 M). The inhibitory effects of morphine and DAMGO were blocked by equimolar concentrations of naloxone and by the selective μ opiate receptor antagonist β-funaltrexamine. Pertussis toxin but not cholera toxin reversed the inhibitory effects of both morphine and DAMGO. Our data suggest that morphine inhibits phagocytosis of C. neoformans by swine microglia via a mechanism involving μ opiate receptors coupled to a pertussis toxin-sensitive G(i)/G(o) protein signaling pathway.

AB - Microglia are important immune effector cells within the brain. The phagocytosis of nonopsonized Cryptococcus neoformans by swine microglia was used as an in vitro model for studies on cellular mechanisms of opiate-mediated immunomodulation in the brain. Morphine inhibited potently (IC50 ~10-16 M) the phagocytosis of C. neoformans by primaty cultures of neonatal pig microglia. The μ opioid agonist Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) also suppressed phagocytosis but with a much lower potency than morphine (IC50 ~ 10-8 M). The inhibitory effects of morphine and DAMGO were blocked by equimolar concentrations of naloxone and by the selective μ opiate receptor antagonist β-funaltrexamine. Pertussis toxin but not cholera toxin reversed the inhibitory effects of both morphine and DAMGO. Our data suggest that morphine inhibits phagocytosis of C. neoformans by swine microglia via a mechanism involving μ opiate receptors coupled to a pertussis toxin-sensitive G(i)/G(o) protein signaling pathway.

KW - Cryptococcus neoformans

KW - microglia

KW - morphine

KW - phagocytosis

KW - μ opiate receptor

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Inhibition of swine microglial cell phagocytosis of Cryptococcus neoformans by femtomolar concentrations of morphine

Abstract

Bibliographical note

Keywords

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