Inhibition of pseudorenin by pepstatin

Rodney L. Johnson; Alan M. Poisner

doi:10.1016/0006-2952(77)90038-7

Inhibition of pseudorenin by pepstatin

Rodney L. Johnson
, Alan M. Poisner

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The pentapeptide pepstatin was found to inhibit the ability of rat spleen pseudorenin to form angiotensin I from tetradecapeptide renin substrate. Dixon and Webb plots showed that this inhibition was noncompetitive in nature. Lineweaver-Burk analysis also showed noncompetitive inhibition. K_i values determined by the three graphical methods ranged from 1.8 to 3.8 × 10^-10M. The K_m for pseudorenin was determined to be between 0.82 and 1.23 μM. The concentration of enzyme used was estimated to be 3.1 × 10^-10M. Pepstatin should prove useful in the future for the analysis and purification of pseudorenin.

Original language	English (US)
Pages (from-to)	639-641
Number of pages	3
Journal	Biochemical Pharmacology
Volume	26
Issue number	7
DOIs	https://doi.org/10.1016/0006-2952(77)90038-7
State	Published - Apr 1 1977

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title = "Inhibition of pseudorenin by pepstatin",

abstract = "The pentapeptide pepstatin was found to inhibit the ability of rat spleen pseudorenin to form angiotensin I from tetradecapeptide renin substrate. Dixon and Webb plots showed that this inhibition was noncompetitive in nature. Lineweaver-Burk analysis also showed noncompetitive inhibition. Ki values determined by the three graphical methods ranged from 1.8 to 3.8 × 10-10M. The Km for pseudorenin was determined to be between 0.82 and 1.23 μM. The concentration of enzyme used was estimated to be 3.1 × 10-10M. Pepstatin should prove useful in the future for the analysis and purification of pseudorenin.",

author = "Johnson, \{Rodney L.\} and Poisner, \{Alan M.\}",

year = "1977",

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doi = "10.1016/0006-2952(77)90038-7",

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T1 - Inhibition of pseudorenin by pepstatin

AU - Johnson, Rodney L.

AU - Poisner, Alan M.

PY - 1977/4/1

Y1 - 1977/4/1

N2 - The pentapeptide pepstatin was found to inhibit the ability of rat spleen pseudorenin to form angiotensin I from tetradecapeptide renin substrate. Dixon and Webb plots showed that this inhibition was noncompetitive in nature. Lineweaver-Burk analysis also showed noncompetitive inhibition. Ki values determined by the three graphical methods ranged from 1.8 to 3.8 × 10-10M. The Km for pseudorenin was determined to be between 0.82 and 1.23 μM. The concentration of enzyme used was estimated to be 3.1 × 10-10M. Pepstatin should prove useful in the future for the analysis and purification of pseudorenin.

AB - The pentapeptide pepstatin was found to inhibit the ability of rat spleen pseudorenin to form angiotensin I from tetradecapeptide renin substrate. Dixon and Webb plots showed that this inhibition was noncompetitive in nature. Lineweaver-Burk analysis also showed noncompetitive inhibition. Ki values determined by the three graphical methods ranged from 1.8 to 3.8 × 10-10M. The Km for pseudorenin was determined to be between 0.82 and 1.23 μM. The concentration of enzyme used was estimated to be 3.1 × 10-10M. Pepstatin should prove useful in the future for the analysis and purification of pseudorenin.

UR - https://www.scopus.com/pages/publications/0017618181

UR - https://www.scopus.com/pages/publications/0017618181#tab=citedBy

U2 - 10.1016/0006-2952(77)90038-7

DO - 10.1016/0006-2952(77)90038-7

M3 - Article

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AN - SCOPUS:0017618181

SN - 0006-2952

VL - 26

SP - 639

EP - 641

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 7

ER -

Inhibition of pseudorenin by pepstatin

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