Inhibition of pseudorenin by pepstatin

Rodney L. Johnson, Alan M. Poisner

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The pentapeptide pepstatin was found to inhibit the ability of rat spleen pseudorenin to form angiotensin I from tetradecapeptide renin substrate. Dixon and Webb plots showed that this inhibition was noncompetitive in nature. Lineweaver-Burk analysis also showed noncompetitive inhibition. Ki values determined by the three graphical methods ranged from 1.8 to 3.8 × 10-10M. The Km for pseudorenin was determined to be between 0.82 and 1.23 μM. The concentration of enzyme used was estimated to be 3.1 × 10-10M. Pepstatin should prove useful in the future for the analysis and purification of pseudorenin.

Original languageEnglish (US)
Pages (from-to)639-641
Number of pages3
JournalBiochemical Pharmacology
Issue number7
StatePublished - Apr 1 1977


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