Inhibition of p65 Nuclear Translocation by Baicalein

Min Bum Seo, Seog Ki Lee, Young Jin Jeon, Jin Su Im

Research output: Contribution to journalShort surveypeer-review

10 Scopus citations

Abstract

We demonstrate that baicalein, a bioactive flavonoid originally isolated from Scutellaria baicalensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of peritoneal macrophages and RAW 264.7 cells with baicalein inhibited LPS-stimulated nitric oxide production in a dose-related manner. Immunohistochemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression in RAW 264.7 cells. Immunostaining of p65, EMSA, and reporter gene assay showed that baicalein inhibited NF-κB nuclear translocation, DNA binding, and transcriptional activation, respectively. Collectively, these series of experiments indicate that baicalein inhibits iNOS gene expression by blocking NF-κB nuclear translocation. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of baicalein on iNOS suggest that baicalein may represent a useful anti-inflammatory agent.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalToxicological Research
Volume27
Issue number2
DOIs
StatePublished - Jun 2011

Keywords

  • Baicalein
  • Inos
  • Macrophages
  • Nf-κb

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