Abstract
Introduction: The vasculature and blood flow in muscle are perturbed in Duchenne muscular dystrophy (DMD) and its mdx mouse model. MicroRNA-92a (miR-92a) is enriched in endothelial cells, especially during ischemic injury. Methods: Because antagonizing miR-92a was shown to result in increased proliferation and migration of endothelial cells and recovery from ischemia, we assessed the effects of Antagomir-92a in vitro in muscle stem cell culture and in vivo in mdx mice. Results: miR-92a was found to be highly expressed in muscle endothelial cells and satellite cells. Treatment with Antagomir-92a increased capillary density and tissue perfusion, which was accompanied by an increase in satellite cells. However, Antagomir-92a–treated mdx mice showed no histological improvement and had worse muscle function. Antagomir-92a suppressed myogenic differentiation in satellite cell culture. Discussion: AntagomiR-92a improves the vasculature but not the muscle in mdx mice, possibly due to its side effects on satellite cell differentiation. Muscle Nerve 59:594–594, 2019.
Original language | English (US) |
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Pages (from-to) | 594-602 |
Number of pages | 9 |
Journal | Muscle and Nerve |
Volume | 59 |
Issue number | 5 |
DOIs | |
State | Published - May 2019 |
Bibliographical note
Publisher Copyright:© 2019 Wiley Periodicals, Inc.
Keywords
- Antagomir
- endothelial cell
- mdx mouse
- miR-92a
- muscular dystrophy
- satellite cell