Inhibition of arsenite-induced apoptosis by aspirin

Nanyue Chen, Wei-Ya Ma, Zigang Dong

Research output: Contribution to journalArticle

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Abstract

Arsenite exposure and subsequent arsenite-induced toxicity and carcinogenesis are common in many countries. Thus the study of chemopreventive compounds that inhibit arsenite-induced toxicity and carcinogenesis is very valuable. In the present work, we investigated the effect of aspirin on arsenite-induced apoptosis and signal transduction by means of luciferase activity, apoptosis analysis and Western blotting. Arsenite induced AP-1 transcriptional activity at the same concentration (20 μM) as was effective for inducing apoptosis. Arsenite-induced apoptosis and AP-1 transactivation in JB6 cells were blocked by aspirin and salicylate (SA). Both aspirin and SA inhibited arsenite-induced phosphorylation of Erks, but had no effect on phosphorylation of JNKs. SA inhibited arsenite-induced phosphorylation of p38 kinase, but aspirin did not. These results indicate that aspirin and SA inhibit arsenite-induced apoptosis through the inhibition of the Erks/AP-1 pathway.

Original languageEnglish (US)
Pages (from-to)3247-3251
Number of pages5
JournalAnticancer Research
Volume21
Issue number5
StatePublished - Dec 1 2001

Keywords

  • AP-1 and MAP kinase
  • Apoptosis
  • Arsenite
  • Aspirin

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    Chen, N., Ma, W-Y., & Dong, Z. (2001). Inhibition of arsenite-induced apoptosis by aspirin. Anticancer Research, 21(5), 3247-3251.