Inheritance of Bacillus thuringiensis Cry2Ab2 protein resistance in Helicoverpa zea (Lepidoptera: Noctuidae)

Fei Yang; Graham P. Head; Paula A. Price; José C. Santiago González; David L. Kerns

doi:10.1002/ps.5916

Inheritance of Bacillus thuringiensis Cry2Ab2 protein resistance in Helicoverpa zea (Lepidoptera: Noctuidae)

Fei Yang, Graham P. Head, Paula A. Price, José C. Santiago González, David L. Kerns

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

BACKGROUND: The corn earworm, Helicoverpa zea (Boddie), is a major target pest of pyramided Bt corn and cotton in the United States. Field-evolved practical resistance to Cry1 and Cry2 proteins in H. zea has been documented in multiple locations in the United States. Understanding the genetic basis of Bt resistance is essential in developing insect resistance management (IRM) strategies for the sustainable use of the Bt crop technology. In this study, we characterized the genetic bases of Cry2Ab2 resistance in H. zea using diet-overlay bioassays with two different forms of Cry2Ab2 protein. RESULTS: Laboratory bioassays using a Cry2Ab2-resistant (RR) strain, a susceptible (SS) strain, as well as cross and backcross strains, revealed that resistance to Cry2Ab2 was autosomally inherited and controlled by more than one locus. In diet bioassays, the dominance of Cry2Ab2 resistance in H. zea varied from incompletely recessive to incompletely dominant across all tested Cry2Ab2 concentrations of either Bt corn leaf powder or solubilized protein. On leaf tissue of TwinLink cotton (expressing Cry1Ab and Cry2Ae), Cry2Ab2 resistance in H. zea was completely dominant. CONCLUSION: These results have significant implications for understanding the widespread field-evolved resistance of H. zea against Cry1 and Cry2 proteins in Bt corn and cotton and should be useful in developing effective IRM strategies for H. zea.

Original language	English (US)
Pages (from-to)	3676-3684
Number of pages	9
Journal	Pest management science
Volume	76
Issue number	11
DOIs	https://doi.org/10.1002/ps.5916
State	Published - Nov 1 2020
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Bayer Crop Science RM1902 and Biotechnology Risk Assessment grant no. 2017-33522-27090/project accession no. 1013740 from the USDA National Institute of Food and Agriculture.

Funding Information:
This work was supported by Bayer Crop Science RM1902 and Biotechnology Risk Assessment grant no. 2017‐33522‐27090/project accession no. 1013740 from the USDA National Institute of Food and Agriculture.

Publisher Copyright:
© 2020 Society of Chemical Industry

Keywords

Bacillus thuringiensis
Cry2Ab2
Helicoverpa zea
inheritance
resistance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/ps.5916

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title = "Inheritance of Bacillus thuringiensis Cry2Ab2 protein resistance in Helicoverpa zea (Lepidoptera: Noctuidae)",

abstract = "BACKGROUND: The corn earworm, Helicoverpa zea (Boddie), is a major target pest of pyramided Bt corn and cotton in the United States. Field-evolved practical resistance to Cry1 and Cry2 proteins in H. zea has been documented in multiple locations in the United States. Understanding the genetic basis of Bt resistance is essential in developing insect resistance management (IRM) strategies for the sustainable use of the Bt crop technology. In this study, we characterized the genetic bases of Cry2Ab2 resistance in H. zea using diet-overlay bioassays with two different forms of Cry2Ab2 protein. RESULTS: Laboratory bioassays using a Cry2Ab2-resistant (RR) strain, a susceptible (SS) strain, as well as cross and backcross strains, revealed that resistance to Cry2Ab2 was autosomally inherited and controlled by more than one locus. In diet bioassays, the dominance of Cry2Ab2 resistance in H. zea varied from incompletely recessive to incompletely dominant across all tested Cry2Ab2 concentrations of either Bt corn leaf powder or solubilized protein. On leaf tissue of TwinLink cotton (expressing Cry1Ab and Cry2Ae), Cry2Ab2 resistance in H. zea was completely dominant. CONCLUSION: These results have significant implications for understanding the widespread field-evolved resistance of H. zea against Cry1 and Cry2 proteins in Bt corn and cotton and should be useful in developing effective IRM strategies for H. zea.",

keywords = "Bacillus thuringiensis, Cry2Ab2, Helicoverpa zea, inheritance, resistance",

author = "Fei Yang and Head, {Graham P.} and Price, {Paula A.} and {Santiago Gonz{\'a}lez}, {Jos{\'e} C.} and Kerns, {David L.}",

note = "Funding Information: This work was supported by Bayer Crop Science RM1902 and Biotechnology Risk Assessment grant no. 2017-33522-27090/project accession no. 1013740 from the USDA National Institute of Food and Agriculture. Funding Information: This work was supported by Bayer Crop Science RM1902 and Biotechnology Risk Assessment grant no. 2017‐33522‐27090/project accession no. 1013740 from the USDA National Institute of Food and Agriculture. Publisher Copyright: {\textcopyright} 2020 Society of Chemical Industry",

year = "2020",

month = nov,

day = "1",

doi = "10.1002/ps.5916",

language = "English (US)",

volume = "76",

pages = "3676--3684",

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AU - Yang, Fei

AU - Head, Graham P.

AU - Price, Paula A.

AU - Santiago González, José C.

AU - Kerns, David L.

N1 - Funding Information: This work was supported by Bayer Crop Science RM1902 and Biotechnology Risk Assessment grant no. 2017-33522-27090/project accession no. 1013740 from the USDA National Institute of Food and Agriculture. Funding Information: This work was supported by Bayer Crop Science RM1902 and Biotechnology Risk Assessment grant no. 2017‐33522‐27090/project accession no. 1013740 from the USDA National Institute of Food and Agriculture. Publisher Copyright: © 2020 Society of Chemical Industry

PY - 2020/11/1

Y1 - 2020/11/1

N2 - BACKGROUND: The corn earworm, Helicoverpa zea (Boddie), is a major target pest of pyramided Bt corn and cotton in the United States. Field-evolved practical resistance to Cry1 and Cry2 proteins in H. zea has been documented in multiple locations in the United States. Understanding the genetic basis of Bt resistance is essential in developing insect resistance management (IRM) strategies for the sustainable use of the Bt crop technology. In this study, we characterized the genetic bases of Cry2Ab2 resistance in H. zea using diet-overlay bioassays with two different forms of Cry2Ab2 protein. RESULTS: Laboratory bioassays using a Cry2Ab2-resistant (RR) strain, a susceptible (SS) strain, as well as cross and backcross strains, revealed that resistance to Cry2Ab2 was autosomally inherited and controlled by more than one locus. In diet bioassays, the dominance of Cry2Ab2 resistance in H. zea varied from incompletely recessive to incompletely dominant across all tested Cry2Ab2 concentrations of either Bt corn leaf powder or solubilized protein. On leaf tissue of TwinLink cotton (expressing Cry1Ab and Cry2Ae), Cry2Ab2 resistance in H. zea was completely dominant. CONCLUSION: These results have significant implications for understanding the widespread field-evolved resistance of H. zea against Cry1 and Cry2 proteins in Bt corn and cotton and should be useful in developing effective IRM strategies for H. zea.

AB - BACKGROUND: The corn earworm, Helicoverpa zea (Boddie), is a major target pest of pyramided Bt corn and cotton in the United States. Field-evolved practical resistance to Cry1 and Cry2 proteins in H. zea has been documented in multiple locations in the United States. Understanding the genetic basis of Bt resistance is essential in developing insect resistance management (IRM) strategies for the sustainable use of the Bt crop technology. In this study, we characterized the genetic bases of Cry2Ab2 resistance in H. zea using diet-overlay bioassays with two different forms of Cry2Ab2 protein. RESULTS: Laboratory bioassays using a Cry2Ab2-resistant (RR) strain, a susceptible (SS) strain, as well as cross and backcross strains, revealed that resistance to Cry2Ab2 was autosomally inherited and controlled by more than one locus. In diet bioassays, the dominance of Cry2Ab2 resistance in H. zea varied from incompletely recessive to incompletely dominant across all tested Cry2Ab2 concentrations of either Bt corn leaf powder or solubilized protein. On leaf tissue of TwinLink cotton (expressing Cry1Ab and Cry2Ae), Cry2Ab2 resistance in H. zea was completely dominant. CONCLUSION: These results have significant implications for understanding the widespread field-evolved resistance of H. zea against Cry1 and Cry2 proteins in Bt corn and cotton and should be useful in developing effective IRM strategies for H. zea.

KW - Bacillus thuringiensis

KW - Cry2Ab2

KW - Helicoverpa zea

KW - inheritance

KW - resistance

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ER -

Inheritance of Bacillus thuringiensis Cry2Ab2 protein resistance in Helicoverpa zea (Lepidoptera: Noctuidae)

Abstract

Bibliographical note

Keywords

UN SDGs

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