Influenza virus detection in the stool of children with acute gastroenteritis

Jianling Xie; Xiao Li Pang; Gillian A.M. Tarr; Yuan Mu; Ran Zhuo; Linda Chui; Bonita E. Lee; Otto G. Vanderkooi; Phillip I. Tarr; Samina Ali; Shannon E. MacDonald; Stephen B. Freedman

doi:10.1016/j.jcv.2020.104565

Influenza virus detection in the stool of children with acute gastroenteritis

Jianling Xie
, Xiao Li Pang
, Gillian A.M. Tarr
, Yuan Mu
, Ran Zhuo
, Linda Chui
, Bonita E. Lee
, Otto G. Vanderkooi
, Phillip I. Tarr
, Samina Ali
, Shannon E. MacDonald
, Stephen B. Freedman

Environmental Health Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

OBJECTIVES: To determine if the clinical characteristics of children with gastroenteritis and influenza identified in their stool differ from those whose stool was influenza-negative.

METHODS: Children <18-years with gastroenteritis whose stool tested negative for enteropathogen were tested for influenza in stool. The clinical features between influenza-positive and influenza-negative gastroenteritis cases were compared. Stools from controls without infection were also tested for influenza.

RESULTS: Among the 440 gastroenteritis cases, those who were influenza test-positive were older [median age 4.0 (IQR: 2.3, 5.5) vs. 1.5 (IQR: 0.5, 4.0) years; P = 0.008], more likely to present in fall or winter (92.3 % vs. 48.0 %; P = 0.001), be febrile (84.6 % vs. 30.6 %; P < 0.001), have respiratory symptoms (91.7 % vs. 44.8 %; P = 0.002), have dehydration [median Clinical Dehydration Scale score: 4 (IQR: 1.5, 4.5) vs. 2 (IQR: 0, 3); P = 0.034], and have higher Modified Vesikari Scale scores [median: 13 (IQR: 10.5, 14.0) vs. 10 (IQR: 9.0, 13.0); P = 0.044], than those who tested negative. Thirteen gastroenteritis cases (13/440; 3.0 %) including one child without respiratory symptoms vs. one control (1/250; 0.4 %) were influenza stool positive.

CONCLUSIONS: Fever, respiratory symptoms, more severe illness, and older age were more common in children with gastroenteritis with influenza detected in stool, compared to those tested negative.

Original language	English (US)
Article number	104565
Journal	Journal of Clinical Virology
Volume	131
DOIs	https://doi.org/10.1016/j.jcv.2020.104565
State	Published - Oct 2020

Bibliographical note

Funding Information:
This research was supported by APPETITE, which is funded by a grant from the Alberta Innovates?Health Solutions Team Collaborative Research Innovation Opportunity. APPETITE is also supported by the Alberta Children's Hospital Research Institute (Calgary, AB, Canada) and the Women and Children's Health Research Institute (Edmonton, AB, Canada), through a Partnership Award. S.B.F was supported by the Alberta Children's Hospital Foundation Professorship in Child Health and Wellness. P.I.T was supported by grant number NIHP30DK052574 (Digestive Diseases Research Core Center). G.A.M.T. was supported by a Canadian Institutes of Health Research Banting Postdoctoral Fellowship, Alberta Innovates Health Programs Postgraduate Fellowship, and University of Calgary Eyes High Postdoctoral Fellowship. The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children's Hospital Foundation.

Funding Information:
This research was supported by APPETITE , which is funded by a grant from the Alberta Innovates–Health Solutions Team Collaborative Research Innovation Opportunity . APPETITE is also supported by the Alberta Children’s Hospital Research Institute (Calgary, AB, Canada) and the Women and Children’s Health Research Institute (Edmonton, AB, Canada) , through a Partnership Award. S.B.F was supported by the Alberta Children’s Hospital Foundation Professorship in Child Health and Wellness . P.I.T was supported by grant number NIH P30DK052574 (Digestive Diseases Research Core Center). G.A.M.T. was supported by a Canadian Institutes of Health Research Banting Postdoctoral Fellowship , Alberta Innovates Health Programs Postgraduate Fellowship , and University of Calgary Eyes High Postdoctoral Fellowship . The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children’s Hospital Foundation .

Publisher Copyright:
© 2020 Elsevier B.V.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Enteropathogens
Gastroenteritis
Influenza
Pediatrics
Reverse transcriptase- quantitative polymerase chain reaction

Publisher link

10.1016/j.jcv.2020.104565

Find It

Find It at U of M Libraries

Cite this

@article{de923bb67a794462818f3f7f1dd91f83,

title = "Influenza virus detection in the stool of children with acute gastroenteritis",

abstract = "OBJECTIVES: To determine if the clinical characteristics of children with gastroenteritis and influenza identified in their stool differ from those whose stool was influenza-negative.METHODS: Children <18-years with gastroenteritis whose stool tested negative for enteropathogen were tested for influenza in stool. The clinical features between influenza-positive and influenza-negative gastroenteritis cases were compared. Stools from controls without infection were also tested for influenza.RESULTS: Among the 440 gastroenteritis cases, those who were influenza test-positive were older [median age 4.0 (IQR: 2.3, 5.5) vs. 1.5 (IQR: 0.5, 4.0) years; P = 0.008], more likely to present in fall or winter (92.3 \% vs. 48.0 \%; P = 0.001), be febrile (84.6 \% vs. 30.6 \%; P < 0.001), have respiratory symptoms (91.7 \% vs. 44.8 \%; P = 0.002), have dehydration [median Clinical Dehydration Scale score: 4 (IQR: 1.5, 4.5) vs. 2 (IQR: 0, 3); P = 0.034], and have higher Modified Vesikari Scale scores [median: 13 (IQR: 10.5, 14.0) vs. 10 (IQR: 9.0, 13.0); P = 0.044], than those who tested negative. Thirteen gastroenteritis cases (13/440; 3.0 \%) including one child without respiratory symptoms vs. one control (1/250; 0.4 \%) were influenza stool positive.CONCLUSIONS: Fever, respiratory symptoms, more severe illness, and older age were more common in children with gastroenteritis with influenza detected in stool, compared to those tested negative.",

keywords = "Enteropathogens, Gastroenteritis, Influenza, Pediatrics, Reverse transcriptase- quantitative polymerase chain reaction",

author = "Jianling Xie and Pang, \{Xiao Li\} and Tarr, \{Gillian A.M.\} and Yuan Mu and Ran Zhuo and Linda Chui and Lee, \{Bonita E.\} and Vanderkooi, \{Otto G.\} and Tarr, \{Phillip I.\} and Samina Ali and MacDonald, \{Shannon E.\} and Freedman, \{Stephen B.\}",

note = "Funding Information: This research was supported by APPETITE, which is funded by a grant from the Alberta Innovates?Health Solutions Team Collaborative Research Innovation Opportunity. APPETITE is also supported by the Alberta Children's Hospital Research Institute (Calgary, AB, Canada) and the Women and Children's Health Research Institute (Edmonton, AB, Canada), through a Partnership Award. S.B.F was supported by the Alberta Children's Hospital Foundation Professorship in Child Health and Wellness. P.I.T was supported by grant number NIHP30DK052574 (Digestive Diseases Research Core Center). G.A.M.T. was supported by a Canadian Institutes of Health Research Banting Postdoctoral Fellowship, Alberta Innovates Health Programs Postgraduate Fellowship, and University of Calgary Eyes High Postdoctoral Fellowship. The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children's Hospital Foundation. Funding Information: This research was supported by APPETITE , which is funded by a grant from the Alberta Innovates–Health Solutions Team Collaborative Research Innovation Opportunity . APPETITE is also supported by the Alberta Children{\textquoteright}s Hospital Research Institute (Calgary, AB, Canada) and the Women and Children{\textquoteright}s Health Research Institute (Edmonton, AB, Canada) , through a Partnership Award. S.B.F was supported by the Alberta Children{\textquoteright}s Hospital Foundation Professorship in Child Health and Wellness . P.I.T was supported by grant number NIH P30DK052574 (Digestive Diseases Research Core Center). G.A.M.T. was supported by a Canadian Institutes of Health Research Banting Postdoctoral Fellowship , Alberta Innovates Health Programs Postgraduate Fellowship , and University of Calgary Eyes High Postdoctoral Fellowship . The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children{\textquoteright}s Hospital Foundation . Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = oct,

doi = "10.1016/j.jcv.2020.104565",

language = "English (US)",

volume = "131",

journal = "Journal of Clinical Virology",

issn = "1386-6532",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Influenza virus detection in the stool of children with acute gastroenteritis

AU - Xie, Jianling

AU - Pang, Xiao Li

AU - Tarr, Gillian A.M.

AU - Mu, Yuan

AU - Zhuo, Ran

AU - Chui, Linda

AU - Lee, Bonita E.

AU - Vanderkooi, Otto G.

AU - Tarr, Phillip I.

AU - Ali, Samina

AU - MacDonald, Shannon E.

AU - Freedman, Stephen B.

N1 - Funding Information: This research was supported by APPETITE, which is funded by a grant from the Alberta Innovates?Health Solutions Team Collaborative Research Innovation Opportunity. APPETITE is also supported by the Alberta Children's Hospital Research Institute (Calgary, AB, Canada) and the Women and Children's Health Research Institute (Edmonton, AB, Canada), through a Partnership Award. S.B.F was supported by the Alberta Children's Hospital Foundation Professorship in Child Health and Wellness. P.I.T was supported by grant number NIHP30DK052574 (Digestive Diseases Research Core Center). G.A.M.T. was supported by a Canadian Institutes of Health Research Banting Postdoctoral Fellowship, Alberta Innovates Health Programs Postgraduate Fellowship, and University of Calgary Eyes High Postdoctoral Fellowship. The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children's Hospital Foundation. Funding Information: This research was supported by APPETITE , which is funded by a grant from the Alberta Innovates–Health Solutions Team Collaborative Research Innovation Opportunity . APPETITE is also supported by the Alberta Children’s Hospital Research Institute (Calgary, AB, Canada) and the Women and Children’s Health Research Institute (Edmonton, AB, Canada) , through a Partnership Award. S.B.F was supported by the Alberta Children’s Hospital Foundation Professorship in Child Health and Wellness . P.I.T was supported by grant number NIH P30DK052574 (Digestive Diseases Research Core Center). G.A.M.T. was supported by a Canadian Institutes of Health Research Banting Postdoctoral Fellowship , Alberta Innovates Health Programs Postgraduate Fellowship , and University of Calgary Eyes High Postdoctoral Fellowship . The Pediatric Emergency Medicine Research Associate Program (PEMRAP) is supported by a grant from the Alberta Children’s Hospital Foundation . Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/10

Y1 - 2020/10

N2 - OBJECTIVES: To determine if the clinical characteristics of children with gastroenteritis and influenza identified in their stool differ from those whose stool was influenza-negative.METHODS: Children <18-years with gastroenteritis whose stool tested negative for enteropathogen were tested for influenza in stool. The clinical features between influenza-positive and influenza-negative gastroenteritis cases were compared. Stools from controls without infection were also tested for influenza.RESULTS: Among the 440 gastroenteritis cases, those who were influenza test-positive were older [median age 4.0 (IQR: 2.3, 5.5) vs. 1.5 (IQR: 0.5, 4.0) years; P = 0.008], more likely to present in fall or winter (92.3 % vs. 48.0 %; P = 0.001), be febrile (84.6 % vs. 30.6 %; P < 0.001), have respiratory symptoms (91.7 % vs. 44.8 %; P = 0.002), have dehydration [median Clinical Dehydration Scale score: 4 (IQR: 1.5, 4.5) vs. 2 (IQR: 0, 3); P = 0.034], and have higher Modified Vesikari Scale scores [median: 13 (IQR: 10.5, 14.0) vs. 10 (IQR: 9.0, 13.0); P = 0.044], than those who tested negative. Thirteen gastroenteritis cases (13/440; 3.0 %) including one child without respiratory symptoms vs. one control (1/250; 0.4 %) were influenza stool positive.CONCLUSIONS: Fever, respiratory symptoms, more severe illness, and older age were more common in children with gastroenteritis with influenza detected in stool, compared to those tested negative.

AB - OBJECTIVES: To determine if the clinical characteristics of children with gastroenteritis and influenza identified in their stool differ from those whose stool was influenza-negative.METHODS: Children <18-years with gastroenteritis whose stool tested negative for enteropathogen were tested for influenza in stool. The clinical features between influenza-positive and influenza-negative gastroenteritis cases were compared. Stools from controls without infection were also tested for influenza.RESULTS: Among the 440 gastroenteritis cases, those who were influenza test-positive were older [median age 4.0 (IQR: 2.3, 5.5) vs. 1.5 (IQR: 0.5, 4.0) years; P = 0.008], more likely to present in fall or winter (92.3 % vs. 48.0 %; P = 0.001), be febrile (84.6 % vs. 30.6 %; P < 0.001), have respiratory symptoms (91.7 % vs. 44.8 %; P = 0.002), have dehydration [median Clinical Dehydration Scale score: 4 (IQR: 1.5, 4.5) vs. 2 (IQR: 0, 3); P = 0.034], and have higher Modified Vesikari Scale scores [median: 13 (IQR: 10.5, 14.0) vs. 10 (IQR: 9.0, 13.0); P = 0.044], than those who tested negative. Thirteen gastroenteritis cases (13/440; 3.0 %) including one child without respiratory symptoms vs. one control (1/250; 0.4 %) were influenza stool positive.CONCLUSIONS: Fever, respiratory symptoms, more severe illness, and older age were more common in children with gastroenteritis with influenza detected in stool, compared to those tested negative.

KW - Enteropathogens

KW - Gastroenteritis

KW - Influenza

KW - Pediatrics

KW - Reverse transcriptase- quantitative polymerase chain reaction

UR - https://www.scopus.com/pages/publications/85089425449

UR - https://www.scopus.com/pages/publications/85089425449#tab=citedBy

U2 - 10.1016/j.jcv.2020.104565

DO - 10.1016/j.jcv.2020.104565

M3 - Article

C2 - 32810839

AN - SCOPUS:85089425449

SN - 1386-6532

VL - 131

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

M1 - 104565

ER -

Influenza virus detection in the stool of children with acute gastroenteritis

Abstract

Bibliographical note

UN SDGs

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this