Influences of circulatory factors on intervertebral disc aging phenotype

Changbin Lei, Debora Colangelo, Prashanti Patil, Vivian Li, Kevin Ngo, Dong Wang, Qing Dong, Matthew J. Yousefzadeh, Hongsheng Lin, Joon Lee, James Kang, Gwendolyn Sowa, Tony Wyss-Coray, Laura J. Niedernhofer, Paul D. Robbins, Derek M. Huffman, Nam Vo

Research output: Contribution to journalArticlepeer-review

Abstract

Whether disc aging is influenced by factors beyond its local environment is an important unresolved question. Here we performed heterochronic parabiosis in mice to study the effects of circulating factors in young and old blood on age-associated intervertebral disc degeneration. Compared to young isochronic pairs (Y-Y), young mice paired with old mice (Y-O) showed significant increases in levels of disc MMP-13 and ADAMTS4, aggrecan fragmentation, and histologic tissue degeneration, but negligible changes in cellular senescence markers (p16INK4a, p21Cip1). Compared to old isochronic pairs (O-O), old mice paired with young mice (O-Y) exhibited a significant decrease in expression of cellular senescence markers (p16, p21, p53), but only marginal decreases in the levels of disc MMP-13 and ADAMTS4, aggrecan fragmentation, and histologic degeneration. Thus, exposing old mice to young blood circulation greatly suppressed disc cellular senescence, but only slightly decreased disc matrix imbalance and degeneration. Conversely, exposing young mice to old blood accelerated their disc matrix imbalance and tissue degeneration, with little effects on disc cellular senescence. Thus, non-cell autonomous effects of circulating factors on disc cellular senescence and matrix homeostasis are complex and suggest that disc matrix homeostasis is modulated by systemic factors and not solely through local disc cellular senescence.

Original languageEnglish (US)
Pages (from-to)12285-12304
Number of pages20
JournalAging
Volume12
Issue number12
DOIs
StatePublished - Jun 30 2020

Bibliographical note

Funding Information:
NV, JL, JK, GS are supported by R01AG044376. PDR and LJN are supported by PO1AG043376, R01AG063543, R56AG059676, R56AG4059675, P01AG062413 and U19 AG056278. DMH is supported by R21AG055026, the American Federation for Aging Research (AFAR), the Einstein Nathan Shock Center (P30AG038072) and the Einstein-Sinai Diabetes Research Center (P30 DK020541).

Keywords

  • Aging
  • Heterochronic parabiosis
  • Intervertebral disc
  • Proteoglycan
  • Systemic factors

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