Influence of pr-M cleavage on the heterogeneity of extracellular dengue virus particles

Jiraphan Junjhon, Thomas J. Edwards, Utaiwan Utaipat, Valorie D. Bowman, Heather A. Holdaway, Wei Zhang, Poonsook Keelapang, Chunya Puttikhunt, Rushika Perera, Paul R. Chipman, Watchara Kasinrerk, Prida Malasit, Richard J. Kuhn, Nopporn Sittisombut

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.

Original languageEnglish (US)
Pages (from-to)8253-8358
Number of pages106
JournalJournal of virology
Volume84
Issue number16
DOIs
StatePublished - Aug 2010

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