Influence of populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium

Jill Gaskell, Amber Marty, Michael Mozuch, Philip J. Kersten, Sandra Splinter BonDurant, Grzegorz Sabat, Ali Azarpira, John Ralph, Oleksandr Skyba, Shawn D. Mansfield, Robert A. Blanchette, Dan Cullen

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Abstract

We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba × tremula) and syringyl (S)-rich transgenic derivatives. A combination of microarrays and liquid chromatography- tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793 proteins. Comparisons of P. chrysosporium transcript abundance in medium containing poplar or glucose as a sole carbon source showed 113 regulated genes, 11 of which were significantly higher (>2-fold, P<0.05) in transgenic line 64 relative to the parental line. Possibly related to the very large amounts of syringyl (S) units in this transgenic tree (94 mol% S), several oxidoreductases were among the upregulated genes. Peptides corresponding to a total of 18 oxidoreductases were identified in medium consisting of biomass from line 64 or 82 (85 mol% S) but not in the parental clone (65 mol% S). These results demonstrate that P. chrysosporium gene expression patterns are substantially influenced by lignin composition.

Original languageEnglish (US)
Pages (from-to)5828-5835
Number of pages8
JournalApplied and environmental microbiology
Volume80
Issue number18
DOIs
StatePublished - 2014

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    Gaskell, J., Marty, A., Mozuch, M., Kersten, P. J., BonDurant, S. S., Sabat, G., Azarpira, A., Ralph, J., Skyba, O., Mansfield, S. D., Blanchette, R. A., & Cullen, D. (2014). Influence of populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium. Applied and environmental microbiology, 80(18), 5828-5835. https://doi.org/10.1128/AEM.01604-14