Influence of physical factors on the growth of insect cells in vitro: II. Sodium and potassium as osmotic pressure regulators of moth cell growth

T. J. Kurtti, Surendra P.S. Chaudhary, Marion A. Brooks

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The tolerances of a cell line (IMC-HZ-1) from a moth, Heliothis zea, for the monovalent cations Na+ and K+ were defined. Cells shifted to media containing more than 70 mm of K+ showed decreased growth rates. No evidence was obtained for Na+ toxicity. The osmotic pressure tolerances were influenced by the K+ concentration of the medium. The richer the medium was in K+, the narrower was the spectrum of osmotic pressure tolerance. Once the limit of K+ tolerance was exceeded, the rate of decline of growth was linear with respect to further increases in K+. This rate of decline was independent of osmotic pressure. The initial responses of cells during one subculture (2 to 4 population doublings) in media differing from the standard medium (used to maintain the cell line) were not reliable indicators of the growth potential of the cells. Continued subculture in such media resulted in an upward trend in population growth rates in most cases.

Original languageEnglish (US)
Pages (from-to)274-285
Number of pages12
JournalIn Vitro: Journal of the Tissue Culture Association
Volume11
Issue number5
DOIs
StatePublished - Oct 1975

Keywords

  • moth cells
  • osmotic pressure
  • potassium
  • sodium

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