Influence of pentobarbital on synaptic plasma membrane protein synthesis

Robert J. Hitzemann; Horace H. Loh

doi:10.1016/0024-3205(77)90125-4

Influence of pentobarbital on synaptic plasma membrane protein synthesis

Robert J. Hitzemann
, Horace H. Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effects of acute and chronic pentobarbital treatment on the incorporation of ³H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.

Original language	English (US)
Pages (from-to)	35-41
Number of pages	7
Journal	Life Sciences
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/0024-3205(77)90125-4
State	Published - Jan 1 1977

Bibliographical note

Funding Information:
Male Sprague-Dawley rats (Simonsen Laboratories, Gilroy, CA .) weighing 180-200 g were implanted with a needle guide above the lateral ventricle. Four to 7 days later, the Snimals were implanted with two placebo or two 200 mg ;This study was supported in part by USPHS Grant DA-OOS64. ent of a NINE Research Scientist Career Development Award,

Publisher link

10.1016/0024-3205(77)90125-4

Find It

Find It at U of M Libraries

Cite this

@article{7facf68854d249068e5534ef27bb1c0c,

title = "Influence of pentobarbital on synaptic plasma membrane protein synthesis",

abstract = "The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.",

author = "Hitzemann, \{Robert J.\} and Loh, \{Horace H.\}",

note = "Funding Information: Male Sprague-Dawley rats (Simonsen Laboratories, Gilroy, CA .) weighing 180-200 g were implanted with a needle guide above the lateral ventricle. Four to 7 days later, the Snimals were implanted with two placebo or two 200 mg ;This study was supported in part by USPHS Grant DA-OOS64. ent of a NINE Research Scientist Career Development Award,",

year = "1977",

month = jan,

day = "1",

doi = "10.1016/0024-3205(77)90125-4",

language = "English (US)",

volume = "20",

pages = "35--41",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "1",

}

TY - JOUR

T1 - Influence of pentobarbital on synaptic plasma membrane protein synthesis

AU - Hitzemann, Robert J.

AU - Loh, Horace H.

N1 - Funding Information: Male Sprague-Dawley rats (Simonsen Laboratories, Gilroy, CA .) weighing 180-200 g were implanted with a needle guide above the lateral ventricle. Four to 7 days later, the Snimals were implanted with two placebo or two 200 mg ;This study was supported in part by USPHS Grant DA-OOS64. ent of a NINE Research Scientist Career Development Award,

PY - 1977/1/1

Y1 - 1977/1/1

N2 - The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.

AB - The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.

UR - https://www.scopus.com/pages/publications/0017349248

UR - https://www.scopus.com/pages/publications/0017349248#tab=citedBy

U2 - 10.1016/0024-3205(77)90125-4

DO - 10.1016/0024-3205(77)90125-4

M3 - Article

C2 - 834110

AN - SCOPUS:0017349248

SN - 0024-3205

VL - 20

SP - 35

EP - 41

JO - Life Sciences

JF - Life Sciences

IS - 1

ER -

Influence of pentobarbital on synaptic plasma membrane protein synthesis

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this