The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.
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Male Sprague-Dawley rats (Simonsen Laboratories, Gilroy, CA .) weighing 180-200 g were implanted with a needle guide above the lateral ventricle. Four to 7 days later, the Snimals were implanted with two placebo or two 200 mg ;This study was supported in part by USPHS Grant DA-OOS64. ent of a NINE Research Scientist Career Development Award,