Influence of microgravity on root-cap regeneration and the structure of columella cells in Zea mays.

R. Moore, C. E. McClelen, W. M. Fondren, C. L. Wang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We launched imbibed seeds and seedlings of Zea mays into outer space aboard the space shuttle Columbia to determine the influence of microgravity on 1) root-cap regeneration, and 2) the distribution of amyloplasts and endoplasmic reticulum (ER) in the putative statocytes (i.e., columella cells) of roots. Decapped roots grown on Earth completely regenerated their caps within 4.8 days after decapping, while those grown in microgravity did not regenerate caps. In Earth-grown seedlings, the ER was localized primarily along the periphery of columella cells, and amyloplasts sedimented in response to gravity to the lower sides of the cells. Seeds germinated on Earth and subsequently launched into outer space had a distribution of ER in columella cells similar to that of Earth-grown controls, but amyloplasts were distributed throughout the cells. Seeds germinated in outer space were characterized by the presence of spherical and ellipsoidal masses of ER and randomly distributed amyloplasts in their columella cells. These results indicate that 1) gravity is necessary for regeneration of the root cap, 2) columella cells can maintain their characteristic distribution of ER in microgravity only if they are exposed previously to gravity, and 3) gravity is necessary to distribute the ER in columella cells of this cultivar of Z. mays.

Original languageEnglish (US)
Pages (from-to)218-223
Number of pages6
JournalAmerican Journal of Botany
Volume74
Issue number2
DOIs
StatePublished - Jan 1 1987

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microgravity
Weightlessness
root cap
cell structures
Zea mays
Regeneration
regeneration
Endoplasmic Reticulum
endoplasmic reticulum
gravity
amyloplasts
Plastids
Gravitation
seed
seedling
cells
Seeds
space shuttle
Seedlings
cultivar

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Influence of microgravity on root-cap regeneration and the structure of columella cells in Zea mays. / Moore, R.; McClelen, C. E.; Fondren, W. M.; Wang, C. L.

In: American Journal of Botany, Vol. 74, No. 2, 01.01.1987, p. 218-223.

Research output: Contribution to journalArticle

Moore, R. ; McClelen, C. E. ; Fondren, W. M. ; Wang, C. L. / Influence of microgravity on root-cap regeneration and the structure of columella cells in Zea mays. In: American Journal of Botany. 1987 ; Vol. 74, No. 2. pp. 218-223.
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