Influence of method of alveolar air collection on results of breath tests

Michael D. Levitt, Carol Ellis, Julie Furne

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The influence of the method alveolar air collection on measurements of trace gas concentration has received little attention. We measured the concentrations of H2, CH4, CO, and CO2 in sequential fractions of alveolar air collected with and without breath-holding. Without breath-holding, the concentration of these gases increased appreciably as increasing quantities of alveolar air were expelled. Twenty seconds of breath-holding markedly reduced this nonhomogeneity of alveolar air. Prediction of the excretion rate of trace gases from measurements of their concentration relative to CO2 and literature values for resting CO2 excretion underestimated the true excretion rate. We conclude that breath-holding prior to sample collection enhances the reproducibility of trace gas measurements. When calculating the rate of excretion of trace gases, the use of literature values for resting ventilation or CO2 excretion may result in appreciable underestimations of the true rate.

Original languageEnglish (US)
Pages (from-to)1938-1945
Number of pages8
JournalDigestive Diseases and Sciences
Issue number9
StatePublished - 1998

Bibliographical note

Funding Information:
Manuscript receive d De ce mber 22, 1997; revise d manuscript rece ived April 29, 1998; accepted May 8, 1998. From the Rese arch Sericev, Minneapolis VA Medical Center, 1 Veterans Drive, Minneapolis, Minnesota 55417. Supported in part by the Departmen t of Veterans Affairs and The National Institute of Diabe tes and Dige stive ad nKidney Diseases Grant R01 DK 13309-25. Address for reprint requests: Dr. Michae l D. Levitt, Mpls VAMC (151), 1 Veterans Drive , Minneapolis, Minnesota 55417.

Copyright 2007 Elsevier B.V., All rights reserved.


  • Breath testing
  • Carbon monoxide
  • Hydrogen
  • Methane


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