Induction of quorum sensing in microdroplets by transporting small molecule through PDMS

Jung Uk Shim; Santoshkumar N. Patil; James T. Hodgkinson; Steven D. Bowden; David R. Spring; Martin Welch; Wilhelm T S Huck; Florian Hollfelder; Chris Abell

Induction of quorum sensing in microdroplets by transporting small molecule through PDMS

Jung Uk Shim, Santoshkumar N. Patil, James T. Hodgkinson, Steven D. Bowden, David R. Spring, Martin Welch, Wilhelm T S Huck, Florian Hollfelder, Chris Abell

Food Science and Nutrition

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

By exploiting the permeability of PDMS fabricated on the microfluidic system, it is possible to deliver hormone-like small molecules into stored microdroplets encapsulating cells, which are harbouring a plasmid to detect quorum sensing molecules. The ability to change the condition of microdroplets provides a tool to investigate responses of individual cells under controllable chemical environments at a given initial condition. This study will be helpful to understand the heterogeneity of cells to different regulators during their lifecycle by simply switching the reservoir conditions in predetermined manners.

Original language	English (US)
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages	656-658
Number of pages	3
Volume	1
State	Published - Dec 1 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: Oct 3 2010 → Oct 7 2010

Other

Other	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/Territory	Netherlands
City	Groningen
Period	10/3/10 → 10/7/10

Keywords

Control of environment
Microdroplet
Permeability of PDMS
Quorum sensing

Find It

Find It at U of M Libraries

Cite this

Induction of quorum sensing in microdroplets by transporting small molecule through PDMS. / Shim, Jung Uk; Patil, Santoshkumar N.; Hodgkinson, James T. et al.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 1 2010. p. 656-658.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shim, JU, Patil, SN, Hodgkinson, JT, Bowden, SD, Spring, DR, Welch, M, Huck, WTS, Hollfelder, F & Abell, C 2010, Induction of quorum sensing in microdroplets by transporting small molecule through PDMS. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 1, pp. 656-658, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/3/10.

@inproceedings{5b1f669352274cda954a1759cea63e47,

title = "Induction of quorum sensing in microdroplets by transporting small molecule through PDMS",

abstract = "By exploiting the permeability of PDMS fabricated on the microfluidic system, it is possible to deliver hormone-like small molecules into stored microdroplets encapsulating cells, which are harbouring a plasmid to detect quorum sensing molecules. The ability to change the condition of microdroplets provides a tool to investigate responses of individual cells under controllable chemical environments at a given initial condition. This study will be helpful to understand the heterogeneity of cells to different regulators during their lifecycle by simply switching the reservoir conditions in predetermined manners.",

keywords = "Control of environment, Microdroplet, Permeability of PDMS, Quorum sensing",

author = "Shim, {Jung Uk} and Patil, {Santoshkumar N.} and Hodgkinson, {James T.} and Bowden, {Steven D.} and Spring, {David R.} and Martin Welch and Huck, {Wilhelm T S} and Florian Hollfelder and Chris Abell",

year = "2010",

month = dec,

day = "1",

language = "English (US)",

isbn = "9781618390622",

volume = "1",

pages = "656--658",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",

}

TY - GEN

T1 - Induction of quorum sensing in microdroplets by transporting small molecule through PDMS

AU - Shim, Jung Uk

AU - Patil, Santoshkumar N.

AU - Hodgkinson, James T.

AU - Bowden, Steven D.

AU - Spring, David R.

AU - Welch, Martin

AU - Huck, Wilhelm T S

AU - Hollfelder, Florian

AU - Abell, Chris

PY - 2010/12/1

Y1 - 2010/12/1

N2 - By exploiting the permeability of PDMS fabricated on the microfluidic system, it is possible to deliver hormone-like small molecules into stored microdroplets encapsulating cells, which are harbouring a plasmid to detect quorum sensing molecules. The ability to change the condition of microdroplets provides a tool to investigate responses of individual cells under controllable chemical environments at a given initial condition. This study will be helpful to understand the heterogeneity of cells to different regulators during their lifecycle by simply switching the reservoir conditions in predetermined manners.

AB - By exploiting the permeability of PDMS fabricated on the microfluidic system, it is possible to deliver hormone-like small molecules into stored microdroplets encapsulating cells, which are harbouring a plasmid to detect quorum sensing molecules. The ability to change the condition of microdroplets provides a tool to investigate responses of individual cells under controllable chemical environments at a given initial condition. This study will be helpful to understand the heterogeneity of cells to different regulators during their lifecycle by simply switching the reservoir conditions in predetermined manners.

KW - Control of environment

KW - Microdroplet

KW - Permeability of PDMS

KW - Quorum sensing

UR - http://www.scopus.com/inward/record.url?scp=84884336000&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884336000&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84884336000

SN - 9781618390622

VL - 1

SP - 656

EP - 658

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

Induction of quorum sensing in microdroplets by transporting small molecule through PDMS

Abstract

Other

Keywords

Find It

Other files and links

Fingerprint

Cite this