Induction of mucous cell metaplasia in the middle ear of rats using a three-step method: An improved model for otitis media with mucoid effusion

Yasuhiro Tsuboi, Youngki Kim, G. Scott Giebink, Chap Le, Michael M. Paparella, Nengqian Chen, Patricia A. Schachern, Steven K. Juhn, Jizhen Lin

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 107 colony-forming units (CFU)/ear or Haemophilus influenzae at 5 × 107 CFU/ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 107 CFU/ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalActa Oto-Laryngologica
Volume122
Issue number2
DOIs
StatePublished - Apr 13 2002

Keywords

  • Middle ear
  • Mucoid effusion
  • Mucous cell metaplasia
  • Otitis media
  • Rats

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