Induction of hepatic synthesis of serum amyloid A protein and actin

J. F. Morrow, R. S. Stearman, C. G. Peltzman, D. A. Potter

Research output: Contribution to journalArticle

119 Scopus citations


Major changes in the mRNA population of murine liver occur after administration of bacterial lipopolysaccharide, an agent that causes increases in the concentrations of acute-phase serum proteins. The mRNA for one of these, serum amyloid A, is increased at least 500-fold compared to the normal level. It becomes one of the most abundant hepatic mRNAs, and serum amyloid A synthesis comprises about 2.5% of total hepatic protein synthesis in the acute-phase response. Its synthesis is tissue-specific in that amyloid A mRNA was not detected in the kidney, an important site of amyloid fibril accumulation. The protein synthesized in largest amount by acute-phase liver tissue in culture is cytoplasmic actin. Its relative rate of synthesis is increased about 5-fold compared to the normal tissue; that of serum albumin is decreased to about one-third of its normal rate. The concentration of mRNA for serum albumin is decreased by a similar amount. Starting with induced liver RNA, we have constructed a recombinant plasmid containing most of the DNA sequence encoding the serum amyloid A polypeptide.

Original languageEnglish (US)
Pages (from-to)4718-4722
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number8 I
StatePublished - 1981
Externally publishedYes

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