Induction of angiogenesis in the β-amyloid peptide-injected rat hippocampus

Ladan Zand, Jae K. Ryu, James G. McLarnon

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Angiogenesis, the formation of new capillary blood vessels, has been studied following the stereotaxic injection of β-amyloid peptide (Aβ1-42) into rat hippocampus. Immunohistochemical analysis for laminin showed that neovascularization was only slightly increased, relative to control, in the hippocampus 1 day post-Aβ1-42 injection. However, 7 days following peptide injection neovascularization was markedly up-regulated (by 2.2-fold) compared to control. Immunoreactivity for the angiogenic stimulatory agent vascular endothelial growth factor (VEGF) was also significantly increased in the hippocampus 7 days after Aβ1-42 injection. Double immunofluorescence staining demonstrated that the increased level of VEGF immunoreactivity was localized to both astrocytes and microglia, suggesting inflammatory responses contributed to angiogenesis. The findings of β-amyloid stimulated angiogenesis and the involvement of peptide-induced inflammatory processes may have relevance to the pathology of Alzheimer's disease.

Original languageEnglish (US)
Pages (from-to)129-132
Number of pages4
Issue number2
StatePublished - Feb 8 2005
Externally publishedYes


  • Alzheimer's disease β-Amyloid peptide
  • Angiogenesis
  • Astrocytes
  • Inflammation
  • Microglia
  • VEGF


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