Inducible targeting of cDCs and their subsets in vivo

Jakob Loschko; Gereon J. Rieke; Heidi A. Schreiber; Matthew M. Meredith; Kai Hui Yao; Pierre Guermonprez; Michel C. Nussenzweig

doi:10.1016/j.jim.2016.04.004

Inducible targeting of cDCs and their subsets in vivo

Jakob Loschko, Gereon J. Rieke, Heidi A. Schreiber, Matthew M. Meredith, Kai Hui Yao, Pierre Guermonprez, Michel C. Nussenzweig

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDC^lSlDTR). cDCs can be specifically depleted by combining zDC^lSlDTR mice with a Csf1r^Cre driver line. In addition, we show that zDC^Cre mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.

Original language	English (US)
Pages (from-to)	32-38
Number of pages	7
Journal	Journal of Immunological Methods
Volume	434
DOIs	https://doi.org/10.1016/j.jim.2016.04.004
State	Published - Jul 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

Dendritic cells
In vivo depletion models
ZDC

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jim.2016.04.004

http://europepmc.org/articles/pmc4902770

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title = "Inducible targeting of cDCs and their subsets in vivo",

abstract = "Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDClSlDTR). cDCs can be specifically depleted by combining zDClSlDTR mice with a Csf1rCre driver line. In addition, we show that zDCCre mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.",

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doi = "10.1016/j.jim.2016.04.004",

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AU - Loschko, Jakob

AU - Rieke, Gereon J.

AU - Schreiber, Heidi A.

AU - Meredith, Matthew M.

AU - Yao, Kai Hui

AU - Guermonprez, Pierre

AU - Nussenzweig, Michel C.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDClSlDTR). cDCs can be specifically depleted by combining zDClSlDTR mice with a Csf1rCre driver line. In addition, we show that zDCCre mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.

AB - Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDClSlDTR). cDCs can be specifically depleted by combining zDClSlDTR mice with a Csf1rCre driver line. In addition, we show that zDCCre mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.

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KW - In vivo depletion models

KW - ZDC

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ER -

Inducible targeting of cDCs and their subsets in vivo

Abstract

Bibliographical note

Keywords

UN SDGs

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