Inducible targeting of cDCs and their subsets in vivo

Jakob Loschko, Gereon J. Rieke, Heidi A. Schreiber, Matthew M. Meredith, Kai Hui Yao, Pierre Guermonprez, Michel C. Nussenzweig

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDClSlDTR). cDCs can be specifically depleted by combining zDClSlDTR mice with a Csf1rCre driver line. In addition, we show that zDCCre mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalJournal of Immunological Methods
Volume434
DOIs
StatePublished - Jul 1 2016

Bibliographical note

Funding Information:
We are grateful to members of the Nussenzweig laboratory for helpful discussion, reagents, or critical reading of the manuscript. This work was supported in part by National Institutes of Health (NIH) grant number AI 13013 . M.C. Nussenzweig is an HHMI investigator.

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

  • Dendritic cells
  • In vivo depletion models
  • ZDC

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