Inducible system for the expression of a putative oncogene, Bcl-6, in eukaryotic cells

G. J. Aviles-Mendoza, Vivian J Bardwell

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosomal abnormalities involving the chromosomal location 3q27 have recently been associated with both diffuse large cell lymphomas and folticl,lar lymphomas. Molecular studies have led to the identification of the Bcl-6 gene at the 3q27 position. Chromosomal abnormalities result in aberrant expression of the BCL-6 protein. The Bcl-6 gene encodes for a zinc-finger protein that acts as a transcriptional repressor. To identify which genes are regulated by BCL-6, inducible expression plasmids were generated using a eukaryotic inducible expression vector, as described by No et at. Using this system, mRNA levels in B cells, which either express or do not express BCL-6, can be compared to identify genes regulated by BCL-6. Since BCL-6 is a transcriptional repressor, we expect to observe down regulation of a specific set of genes when BCL-6 is present. A better understanding of the role of aberrant BCL-6 in the generation of diffuse large cell lymphoma will be achived through the identification of genes regulated by BCL-6. The funding of this research came from the Undergraduate Research Program from the University of Minnesota.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997

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