Chromosomal abnormalities involving the chromosomal location 3q27 have recently been associated with both diffuse large cell lymphomas and folticl,lar lymphomas. Molecular studies have led to the identification of the Bcl-6 gene at the 3q27 position. Chromosomal abnormalities result in aberrant expression of the BCL-6 protein. The Bcl-6 gene encodes for a zinc-finger protein that acts as a transcriptional repressor. To identify which genes are regulated by BCL-6, inducible expression plasmids were generated using a eukaryotic inducible expression vector, as described by No et at. Using this system, mRNA levels in B cells, which either express or do not express BCL-6, can be compared to identify genes regulated by BCL-6. Since BCL-6 is a transcriptional repressor, we expect to observe down regulation of a specific set of genes when BCL-6 is present. A better understanding of the role of aberrant BCL-6 in the generation of diffuse large cell lymphoma will be achived through the identification of genes regulated by BCL-6. The funding of this research came from the Undergraduate Research Program from the University of Minnesota.
|Original language||English (US)|
|State||Published - Dec 1 1997|