Inducible nitric oxide synthase expression in porcine immune cells

Mary S. Pampusch; Antoinette M. Bennaars; Stefan Harsch; Michael P. Murtaugh

doi:10.1016/S0165-2427(97)00139-6

Inducible nitric oxide synthase expression in porcine immune cells

Mary S. Pampusch, Antoinette M. Bennaars, Stefan Harsch, Michael P. Murtaugh

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Porcine immune cells were examined for the ability to produce inducible nitric oxide synthase following in vitro or in vivo stimulation. Enzyme activity and product formation were not detected following stimulation of porcine peripheral blood mononuclear cells (PBMC), splenocytes, or alveolar macrophages with a combination of ConA and lipopolysaccharide (LPS) or recombinant porcine interferon γ, and LPS. In vitro engulfment of Haemophilus parasuis by macrophages also failed to induce inducible nitric oxide synthase (iNOS) activity or nitrite formation. Swine Herpes Virus infection led to a small but significant increase in level of nitrite detected in lung lavage fluid, whereas the infection of pigs with Porcine Respiratory and Reproductive Syndrome Virus did not alter the lavage fluid nitrite levels. iNOS mRNA was detected in both stimulated and unstimulated porcine immune cells and in macrophages from both control and infected animals suggesting that it is constitutively expressed with little or no upregulation following cellular stimulation. The results presented in this paper indicate that the reactive nitrogen intermediate pathway is not an vital innate immune response in the pig.

Original language	English (US)
Pages (from-to)	279-289
Number of pages	11
Journal	Veterinary immunology and immunopathology
Volume	61
Issue number	2-4
DOIs	https://doi.org/10.1016/S0165-2427(97)00139-6
State	Published - Feb 27 1998

Bibliographical note

Funding Information:
The authors would like to thank Dr. Gloria Solano for assistance with PRRS infection, and H. parasuis engulfment studies, Dr. Jack Risdahl for providing lavage fluid and macrophages from SHV-1 and morphine-treated pigs (funding provided by NIDA grant #K20DA00188-02), the laboratory of Mark Rutherford for assistance with isolation and culture of murine bone marrow macrophages, and the laboratory of Dr. Esam El-Fakawany for assistance with the enzyme assay development and provision of the neuroblastoma cells used as a positive control. Funding for this work was provided by Minnesota AES grant 63-027. MSP was funded by a Training in Psychoneuroimmunology and Substance Abuse grant (NIDA grant #DA-07239), AMB was funded by the NSF-REU program at the University of Minnesota.

Keywords

Macrophages
Morphine
Nitric oxide
Pig
Splenocytes
Swine

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title = "Inducible nitric oxide synthase expression in porcine immune cells",

abstract = "Porcine immune cells were examined for the ability to produce inducible nitric oxide synthase following in vitro or in vivo stimulation. Enzyme activity and product formation were not detected following stimulation of porcine peripheral blood mononuclear cells (PBMC), splenocytes, or alveolar macrophages with a combination of ConA and lipopolysaccharide (LPS) or recombinant porcine interferon γ, and LPS. In vitro engulfment of Haemophilus parasuis by macrophages also failed to induce inducible nitric oxide synthase (iNOS) activity or nitrite formation. Swine Herpes Virus infection led to a small but significant increase in level of nitrite detected in lung lavage fluid, whereas the infection of pigs with Porcine Respiratory and Reproductive Syndrome Virus did not alter the lavage fluid nitrite levels. iNOS mRNA was detected in both stimulated and unstimulated porcine immune cells and in macrophages from both control and infected animals suggesting that it is constitutively expressed with little or no upregulation following cellular stimulation. The results presented in this paper indicate that the reactive nitrogen intermediate pathway is not an vital innate immune response in the pig.",

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note = "Funding Information: The authors would like to thank Dr. Gloria Solano for assistance with PRRS infection, and H. parasuis engulfment studies, Dr. Jack Risdahl for providing lavage fluid and macrophages from SHV-1 and morphine-treated pigs (funding provided by NIDA grant #K20DA00188-02), the laboratory of Mark Rutherford for assistance with isolation and culture of murine bone marrow macrophages, and the laboratory of Dr. Esam El-Fakawany for assistance with the enzyme assay development and provision of the neuroblastoma cells used as a positive control. Funding for this work was provided by Minnesota AES grant 63-027. MSP was funded by a Training in Psychoneuroimmunology and Substance Abuse grant (NIDA grant #DA-07239), AMB was funded by the NSF-REU program at the University of Minnesota.",

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AU - Bennaars, Antoinette M.

AU - Harsch, Stefan

AU - Murtaugh, Michael P.

N1 - Funding Information: The authors would like to thank Dr. Gloria Solano for assistance with PRRS infection, and H. parasuis engulfment studies, Dr. Jack Risdahl for providing lavage fluid and macrophages from SHV-1 and morphine-treated pigs (funding provided by NIDA grant #K20DA00188-02), the laboratory of Mark Rutherford for assistance with isolation and culture of murine bone marrow macrophages, and the laboratory of Dr. Esam El-Fakawany for assistance with the enzyme assay development and provision of the neuroblastoma cells used as a positive control. Funding for this work was provided by Minnesota AES grant 63-027. MSP was funded by a Training in Psychoneuroimmunology and Substance Abuse grant (NIDA grant #DA-07239), AMB was funded by the NSF-REU program at the University of Minnesota.

PY - 1998/2/27

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N2 - Porcine immune cells were examined for the ability to produce inducible nitric oxide synthase following in vitro or in vivo stimulation. Enzyme activity and product formation were not detected following stimulation of porcine peripheral blood mononuclear cells (PBMC), splenocytes, or alveolar macrophages with a combination of ConA and lipopolysaccharide (LPS) or recombinant porcine interferon γ, and LPS. In vitro engulfment of Haemophilus parasuis by macrophages also failed to induce inducible nitric oxide synthase (iNOS) activity or nitrite formation. Swine Herpes Virus infection led to a small but significant increase in level of nitrite detected in lung lavage fluid, whereas the infection of pigs with Porcine Respiratory and Reproductive Syndrome Virus did not alter the lavage fluid nitrite levels. iNOS mRNA was detected in both stimulated and unstimulated porcine immune cells and in macrophages from both control and infected animals suggesting that it is constitutively expressed with little or no upregulation following cellular stimulation. The results presented in this paper indicate that the reactive nitrogen intermediate pathway is not an vital innate immune response in the pig.

AB - Porcine immune cells were examined for the ability to produce inducible nitric oxide synthase following in vitro or in vivo stimulation. Enzyme activity and product formation were not detected following stimulation of porcine peripheral blood mononuclear cells (PBMC), splenocytes, or alveolar macrophages with a combination of ConA and lipopolysaccharide (LPS) or recombinant porcine interferon γ, and LPS. In vitro engulfment of Haemophilus parasuis by macrophages also failed to induce inducible nitric oxide synthase (iNOS) activity or nitrite formation. Swine Herpes Virus infection led to a small but significant increase in level of nitrite detected in lung lavage fluid, whereas the infection of pigs with Porcine Respiratory and Reproductive Syndrome Virus did not alter the lavage fluid nitrite levels. iNOS mRNA was detected in both stimulated and unstimulated porcine immune cells and in macrophages from both control and infected animals suggesting that it is constitutively expressed with little or no upregulation following cellular stimulation. The results presented in this paper indicate that the reactive nitrogen intermediate pathway is not an vital innate immune response in the pig.

KW - Macrophages

KW - Morphine

KW - Nitric oxide

KW - Pig

KW - Splenocytes

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ER -

Inducible nitric oxide synthase expression in porcine immune cells

Abstract

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Keywords

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