Inducible cassette exchange: A rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells

Michelina Iacovino, Darko Bosnakovski, Holger Fey, Danielle Rux, Gagan Bajwa, Elisabeth Mahen, Ana Mitanoska, Zhaohui Xu, Michael Kyba

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

Genetic modification is critically enabling for studies addressing specification and maintenance of cell fate; however, methods for engineering modifications are inefficient. We demonstrate a rapid and efficient recombination system in which an inducible, floxed cre allele replaces itself with an incoming transgene. We target this inducible cassette exchange (ICE) allele to the (HPRT) locus and demonstrate recombination in murine embryonic stem cells (ESCs) and primary cells from derivative ICE mice. Using lentivectors, we demonstrate recombination at a randomly integrated ICE locus in human ESCs. To illustrate the utility of this system, we insert the myogenic regulator, Myf5, into the ICE locus in each platform. This enables efficient directed differentiation of mouse and human ESCs into skeletal muscle and conditional myogenic transdetermination of primary cells cultured in vitro. This versatile tool is thus well suited to gain-of-function studies probing gene function in the specification and reprogramming of cell fate.

Original languageEnglish (US)
Pages (from-to)1580-1588
Number of pages9
JournalSTEM CELLS
Volume29
Issue number10
DOIs
StatePublished - Oct 2011

Keywords

  • Cassette exchange recombination
  • Conditional gene expression
  • ESCs

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