Indolent B-Lineage Precursor Populations Identified by Flow Cytometry and Immunohistochemistry in Benign Lymph Nodes

Andrew B. Stone, Bartosz J. Grzywacz, Elizabeth L Courville, Michael A. Linden

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Objectives: In this retrospective study, we report a series of benign lymph nodes showing small populations of normal B-cell precursors characterized by flow cytometry and immunohistochemistry. Methods: Ten cases identified during clinical flow cytometry practice were retrospectively reanalyzed with particular attention to hematogone categorization and enumeration. Immunohistochemical staining was performed on five excisional lymph node biopsy specimens to characterize the morphologic correlate. Results: Populations of hematogones ranging from 0.13% to 1.86% (median, 0.51%) of all viable leukocytes were demonstrated in 10 benign lymph node samples from eight different patients ranging in age from 17 to 45 years (median, 37.5). These hematogones showed a characteristic immunophenotype (CD19+/CD10+) and maturational pattern by flow cytometry, with progression from stage 1 (median, 0.03%) to stage 2 (median, 0.19%) to stage 3 (median, 0.26%) seen in all cases. Immunohistochemical staining on five excisional biopsy specimens demonstrated a distinct perisinusoidal distribution of CD10+/CD20+cells with a subset of TdT+cells, providing a morphologic correlate. Conclusions: To our knowledge, this is the first study to characterize distinct hematogone populations within benign lymph nodes by both flow cytometry and immunohistochemistry. Recognizing these normal B-cell precursor populations is important to avoid their miscategorization as a CD10+ B-cell neoplasm.

Original languageEnglish (US)
Pages (from-to)202-211
Number of pages10
JournalAmerican journal of clinical pathology
Issue number2
StatePublished - Feb 1 2022

Bibliographical note

Publisher Copyright:
© 2021 American Society for Clinical Pathology,. All rights reserved.For permissions, please e-mail:


  • B-lineage precursors
  • Flow cytometry
  • Hematogones
  • Immunophenotype
  • Lymph nodes
  • Lymphoid hyperplasia

PubMed: MeSH publication types

  • Journal Article


Dive into the research topics of 'Indolent B-Lineage Precursor Populations Identified by Flow Cytometry and Immunohistochemistry in Benign Lymph Nodes'. Together they form a unique fingerprint.

Cite this