Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana (L.) Heynh. glucosinolate mutants and the development of clubroot disease

Jutta Ludwig-Müller, Kerstin Pieper, Manfred Ruppel, Jerry D. Cohen, Ephraim Epstein, Guy Kiddle, Richard Bennett

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Mutants and wild type plants of Arabidopsis thaliana were analysed for differences in glucosinolate accumulation patterns, indole-3-acetic acid (IAA) biosynthesis and phenotype. A previously identified series of mutants, termed TU, with altered glucosinolate patterns was used in this study. Only the line TU8 was affected in shoot phenotype (shorter stems, altered branching pattern). Synthesis of IAA and metabolism were not much affected in the TU8 mutant during seedling development, although the content of free IAA peaked earlier in TUff during plant development than in the wild type. Indole glucosinolates and IAA may, however, be involved in the development of clubroot disease caused by the obligate biotrophic fungus Plasmodiophora brassicae since the TU3 line had a lower infection rate than the wild type and lines TU3 and TU8 showed decreased symptom development The decline in clubroot formation was accompanied by a reduced number of fungal structures within the root cortex and slower development of the fungus. Indole glucosinolates were lower in infected roots of TU3 and TU8 than in control roots of these lines, whereas in wild-type plants the differences were not as prominent. Free IAA and indole-3-acetonitrile (IAN) were increased in infected roots of the wild type and mutants with normal clubroot symptoms, whereas they were reduced in infected roots of mutants TU3 and TU8. These results indicate a role for indole glucosinolates and IAN, IAA in relation to symptom development in clubroot disease.

Original languageEnglish (US)
Pages (from-to)409-419
Number of pages11
JournalPlanta
Volume208
Issue number3
DOIs
StatePublished - Jan 1 1999

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Glucosinolates
Indoleacetic Acids
indoles
glucosinolates
Arabidopsis
indole acetic acid
auxins
Arabidopsis thaliana
biosynthesis
mutants
signs and symptoms (plants)
Fungi
Plasmodiophorida
Fungal Structures
Plasmodiophora brassicae
Phenotype
tuff
phenotype
fungi
Plant Development

Keywords

  • Arabidopsis
  • Auxin
  • Clubroot disease
  • Glucosinolate
  • Plasmodiophora

Cite this

Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana (L.) Heynh. glucosinolate mutants and the development of clubroot disease. / Ludwig-Müller, Jutta; Pieper, Kerstin; Ruppel, Manfred; Cohen, Jerry D.; Epstein, Ephraim; Kiddle, Guy; Bennett, Richard.

In: Planta, Vol. 208, No. 3, 01.01.1999, p. 409-419.

Research output: Contribution to journalArticle

Ludwig-Müller, Jutta ; Pieper, Kerstin ; Ruppel, Manfred ; Cohen, Jerry D. ; Epstein, Ephraim ; Kiddle, Guy ; Bennett, Richard. / Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana (L.) Heynh. glucosinolate mutants and the development of clubroot disease. In: Planta. 1999 ; Vol. 208, No. 3. pp. 409-419.
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