Indocyanine-green-embedded PEBBLEs as a contrast agent for photoacoustic imaging

Gwangseong Kim, Sheng Wen Huang, Kathleen C. Day, Matthew O'Donnell, Rodney R. Agayan, Mark A. Day, Raoul Kopelman, Shai Ashkenazi

Research output: Contribution to journalArticlepeer-review

161 Scopus citations

Abstract

Nanoparticles 100 nm in diameter containing indocyanine green (ICG) have been developed as a contrast agent for photoacoustic (PA) imaging based on (photonic explorers for biomedical use by biologically localized embedding PEBBLE) technology using organically modified silicate (ormosil) as a matrix. ICG is an FDA-approved dye with strong optical absorption in the near-infrared (NIR) region, where light can penetrate deepest into biological tissue. A photoacoustic imaging system was used to study image contrast as a function of PEBBLE concentration in phantom objects. ICG-embedded ormosil PEBBLEs showed improved stability in aqueous solution compared with free ICG dye. The particles were conjugated with HER-2 antibody for breast cancer and prostate cancer cell targeting. Initial in vitro characterization shows high contrast and high efficiency for binding to prostate cancer cells. ICG can also be used as a photosensitizer (generating toxic oxygen by illumination) for photodynamic therapy. We have measured the photosensitization capability of ICGembedded ormosil nanoparticles. This feature can be utilized to combine detection and therapeutic functions in a single agent.

Original languageEnglish (US)
Article number044020
JournalJournal of biomedical optics
Volume12
Issue number4
DOIs
StatePublished - Jul 2007

Keywords

  • Cell targeting
  • Indocyanine green (ICG)
  • Photoacoustic (PA) imaging
  • Photonic explorers for biomedical use by biologically localized embedding (PEBBLE)

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