Indirect evidence for separate vesicular neuronal origins of norepinephrine and ATP in the rabbit vas deferens

George J. Trachte; S. Bruce Binder; Michael J. Peach

doi:10.1016/0014-2999(89)90250-1

Indirect evidence for separate vesicular neuronal origins of norepinephrine and ATP in the rabbit vas deferens

George J. Trachte, S. Bruce Binder, Michael J. Peach

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Abstract

Various modulators of neurotransmission were examined for selective effects on the non-adrenergic or adrenergic components of neurotransmission in the vas deferens to test the hypothesis that ATP and norepinephrine are secreted from the same vesicles. The ATP receptor antagonist, arylazido aminopropionyl ATP (ANAPP), selectively depressed the non-adrenergic contraction and prazosin selectivity depressed the adrenergic contraction in response to electrical stimulation. These results are consistent with the presence of two neurotransmitters, ATP and norepinephrine, which mediate neurogenic contractions. Prostaglandin E₂ inhibited non-adrenergic, but enhanced adrenergic, electrically induced (10 Hz) contractions, presumably via a prejunctional mechanism. The adrenergic component of the neurogenic response was significantly more sensitive to treatment with guanethidine, guanabenz, and 6-hydroxydopamine. These results with a variety of agents are inconsistent with the hypothesis that the neurotransmitters, ATP and norepineprine, are released in tandem from the same neuronal granules.

Original language	English (US)
Pages (from-to)	425-433
Number of pages	9
Journal	European Journal of Pharmacology
Volume	164
Issue number	3
DOIs	https://doi.org/10.1016/0014-2999(89)90250-1
State	Published - May 30 1989
Externally published	Yes

Bibliographical note

Funding Information:
We thank Robert Marszalek for his expert technical assistance, Mr. Charles Brownley, Jr. for the supply of guanethidine sulfate and Ms. Susan Kurki and Diane Peplinski for the manuscript preparation. This work was supported by Grants RO1 HL19242 and RO1 HL34600 from the National Heart, Lung and Blood Institute.

Keywords

(Neuromodulators)
Adrenergic neurotransmission
Guanabenz
Guanethidine
PGE
Purinergic neurotransmission

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10.1016/0014-2999(89)90250-1

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title = "Indirect evidence for separate vesicular neuronal origins of norepinephrine and ATP in the rabbit vas deferens",

abstract = "Various modulators of neurotransmission were examined for selective effects on the non-adrenergic or adrenergic components of neurotransmission in the vas deferens to test the hypothesis that ATP and norepinephrine are secreted from the same vesicles. The ATP receptor antagonist, arylazido aminopropionyl ATP (ANAPP), selectively depressed the non-adrenergic contraction and prazosin selectivity depressed the adrenergic contraction in response to electrical stimulation. These results are consistent with the presence of two neurotransmitters, ATP and norepinephrine, which mediate neurogenic contractions. Prostaglandin E2 inhibited non-adrenergic, but enhanced adrenergic, electrically induced (10 Hz) contractions, presumably via a prejunctional mechanism. The adrenergic component of the neurogenic response was significantly more sensitive to treatment with guanethidine, guanabenz, and 6-hydroxydopamine. These results with a variety of agents are inconsistent with the hypothesis that the neurotransmitters, ATP and norepineprine, are released in tandem from the same neuronal granules.",

keywords = "(Neuromodulators), Adrenergic neurotransmission, Guanabenz, Guanethidine, PGE, Purinergic neurotransmission",

author = "Trachte, {George J.} and {Bruce Binder}, S. and Peach, {Michael J.}",

note = "Funding Information: We thank Robert Marszalek for his expert technical assistance, Mr. Charles Brownley, Jr. for the supply of guanethidine sulfate and Ms. Susan Kurki and Diane Peplinski for the manuscript preparation. This work was supported by Grants RO1 HL19242 and RO1 HL34600 from the National Heart, Lung and Blood Institute.",

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doi = "10.1016/0014-2999(89)90250-1",

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AU - Trachte, George J.

AU - Bruce Binder, S.

AU - Peach, Michael J.

N1 - Funding Information: We thank Robert Marszalek for his expert technical assistance, Mr. Charles Brownley, Jr. for the supply of guanethidine sulfate and Ms. Susan Kurki and Diane Peplinski for the manuscript preparation. This work was supported by Grants RO1 HL19242 and RO1 HL34600 from the National Heart, Lung and Blood Institute.

PY - 1989/5/30

Y1 - 1989/5/30

N2 - Various modulators of neurotransmission were examined for selective effects on the non-adrenergic or adrenergic components of neurotransmission in the vas deferens to test the hypothesis that ATP and norepinephrine are secreted from the same vesicles. The ATP receptor antagonist, arylazido aminopropionyl ATP (ANAPP), selectively depressed the non-adrenergic contraction and prazosin selectivity depressed the adrenergic contraction in response to electrical stimulation. These results are consistent with the presence of two neurotransmitters, ATP and norepinephrine, which mediate neurogenic contractions. Prostaglandin E2 inhibited non-adrenergic, but enhanced adrenergic, electrically induced (10 Hz) contractions, presumably via a prejunctional mechanism. The adrenergic component of the neurogenic response was significantly more sensitive to treatment with guanethidine, guanabenz, and 6-hydroxydopamine. These results with a variety of agents are inconsistent with the hypothesis that the neurotransmitters, ATP and norepineprine, are released in tandem from the same neuronal granules.

AB - Various modulators of neurotransmission were examined for selective effects on the non-adrenergic or adrenergic components of neurotransmission in the vas deferens to test the hypothesis that ATP and norepinephrine are secreted from the same vesicles. The ATP receptor antagonist, arylazido aminopropionyl ATP (ANAPP), selectively depressed the non-adrenergic contraction and prazosin selectivity depressed the adrenergic contraction in response to electrical stimulation. These results are consistent with the presence of two neurotransmitters, ATP and norepinephrine, which mediate neurogenic contractions. Prostaglandin E2 inhibited non-adrenergic, but enhanced adrenergic, electrically induced (10 Hz) contractions, presumably via a prejunctional mechanism. The adrenergic component of the neurogenic response was significantly more sensitive to treatment with guanethidine, guanabenz, and 6-hydroxydopamine. These results with a variety of agents are inconsistent with the hypothesis that the neurotransmitters, ATP and norepineprine, are released in tandem from the same neuronal granules.

KW - (Neuromodulators)

KW - Adrenergic neurotransmission

KW - Guanabenz

KW - Guanethidine

KW - PGE

KW - Purinergic neurotransmission

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Indirect evidence for separate vesicular neuronal origins of norepinephrine and ATP in the rabbit vas deferens

Abstract

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