Increasing NADPH impairs fungal H2O2 resistance by perturbing transcriptional regulation of peroxiredoxin

Jingyi Li, Yanwei Sun, Feiyun Liu, Yao Zhou, Yunfeng Yan, Zhemin Zhou, Ping Wang, Shengmin Zhou

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

NADPH provides the reducing power for decomposition of reactive oxygen species (ROS), making it an indispensable part during ROS defense. It remains uncertain, however, if living cells respond to the ROS challenge with an elevated intracellular NADPH level or a more complex NADPH-mediated manner. Herein, we employed a model fungus Aspergillus nidulans to probe this issue. A conditional expression of glucose-6-phosphate dehydrogenase (G6PD)-strain was constructed to manipulate intracellular NADPH levels. As expected, turning down the cellular NADPH concentration drastically lowered the ROS response of the strain; it was interesting to note that increasing NADPH levels also impaired fungal H2O2 resistance. Further analysis showed that excess NADPH promoted the assembly of the CCAAT-binding factor AnCF, which in turn suppressed NapA, a transcriptional activator of PrxA (the key NADPH-dependent ROS scavenger), leading to low antioxidant ability. In natural cell response to oxidative stress, we noticed that the intracellular NADPH level fluctuated “down then up” in the presence of H2O2. This might be the result of a co-action of the PrxA-dependent NADPH consumption and NADPH-dependent feedback of G6PD. The fluctuation of NADPH is well correlated to the formation of AnCF assembly and expression of NapA, thus modulating the ROS defense. Our research elucidated how A. nidulans precisely controls NADPH levels for ROS defense. Graphical Abstract: [Figure not available: see fulltext.].

Original languageEnglish (US)
Article number1
JournalBioresources and Bioprocessing
Volume9
Issue number1
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
This study was supported by the International S&T Innovation Cooperation Key Project (2017YFE0129600), the National Natural Science Foundation of China (21672065, 22077032 and 21636003), the National Major Science and Technology Projects of China (2019ZX09739001), the Fundamental Research Funds for the Central Universities (22221818014), and the 111 Project (B18022).

Publisher Copyright:
© 2021, The Author(s).

Keywords

  • AnCF
  • Aspergillus
  • Glucose-6-phosphate dehydrogenase
  • NADPH
  • Oxidative stress
  • Peroxiredoxin

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