Increased ribonucleotide reductase activity in hydroxyurea-resistant mosquito cells

Anna Gerenday, Karen M. Shih, Carter C. Herman, Ann M. Fallon

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Hydroxyurea-resistant Aedes albopictus mosquito cells were selected by incremental exposure of unmutagenized cells to hydroxyurea concentrations ranging from 0.1 to 8 mM. Clonal populations that had become 40-fold more resistant to hydroxyurea than wild-type cells varied in morphology, and their growth rate decreased to a ∼45 h doubling time, relative to an 18 h doubling time in unselected cells. At this level of resistance, the cells remained diploid, with a modal chromosome number of 6. When labelled with 35S[methionine/cysteine], clone HU1062, which grew in the presence of 8 mM hydroxyurea, overproduced a labeled protein with the approximate size of the 45,000 dalton M2 subunit of ribonucleotide reductase. Consistent with this observation, ribonucleotide reductase activity in HU-1062 cells was approximately 10-fold higher than in wild-type control cells. This is the first example of an hydroxyurea-resistant insect cell line.

Original languageEnglish (US)
Pages (from-to)31-41
Number of pages11
JournalArchives of Insect Biochemistry and Physiology
Volume34
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • Aedes albopictus
  • Hydroxyurea-resistant cell line
  • Ribonucleotide

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