Abstract
Hydroxyurea-resistant Aedes albopictus mosquito cells were selected by incremental exposure of unmutagenized cells to hydroxyurea concentrations ranging from 0.1 to 8 mM. Clonal populations that had become 40-fold more resistant to hydroxyurea than wild-type cells varied in morphology, and their growth rate decreased to a ∼45 h doubling time, relative to an 18 h doubling time in unselected cells. At this level of resistance, the cells remained diploid, with a modal chromosome number of 6. When labelled with 35S[methionine/cysteine], clone HU1062, which grew in the presence of 8 mM hydroxyurea, overproduced a labeled protein with the approximate size of the 45,000 dalton M2 subunit of ribonucleotide reductase. Consistent with this observation, ribonucleotide reductase activity in HU-1062 cells was approximately 10-fold higher than in wild-type control cells. This is the first example of an hydroxyurea-resistant insect cell line. Arch. Insect Biochem. Physiol. 34:31-41, 1997.
Original language | English (US) |
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Pages (from-to) | 19-25 |
Number of pages | 7 |
Journal | Archives of Insect Biochemistry and Physiology |
Volume | 46 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 2001 |
Keywords
- Aedes albopictus
- Hydroxyurea-resistant cell line
- Ribonucleotide